A semi-quantitative dipstick assay for microcystin

authored by: Nils Tippkötter, Henning Stückmann, Stephen Kroll, Gunda Winkelmann, Udo Noack, Thomas Scheper, Roland Ulber
Abstract: An immunochromatographic lateral flow dipstick assay for the fast detection of microcystin-LR was developed. Colloid gold particles with diameters of 40 nm were used as red-colored antibody labels for the visual detection of the antigen. The new dipstick sensor is capable of detecting down to 5 μg•l^-1 (ppb; total inversion of the color signal) or 1 ppb (observation of color grading) of microcystin-LR. The course of the labeling reaction was observed via spectrometric wave shifts caused by the change of particle size during the binding of antibodies. Different stabilizing reagents showed that especially bovine serum albumin (BSA) and casein increase the assays sensitivity and the conjugate stability. Performance of the dipsticks was quantified by pattern processing of capture zone CCD images. Storage stability of dipsticks and conjugate suspensions over 115 days under different conditions were monitored. The ready-to-use dipsticks were successfully tested with microcystin-LR-spiked samples of outdoor drinking- and salt water and applied to the tissue of microcystin-fed mussels.
Organisation(s): Institute of Technical Chemistry
External Organisation(s): University of Kaiserslautern
Noack Laboratorien GmbH
Type: Article
Journal: Analytical and Bioanalytical Chemistry
Volume: 394
Pages: 863-869
No. of pages: 7
ISSN: 1618-2642
Publication date: 24.03.2009
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Analytical Chemistry, Biochemistry
Electronic version(s): https://doi.org/10.1007/s00216-009-2750-8 (Access: Unknown)

BibTeX

@article{f3eb24fd2add48849fd978c5a19f5e63,
title = "A semi-quantitative dipstick assay for microcystin",
abstract = "An immunochromatographic lateral flow dipstick assay for the fast detection of microcystin-LR was developed. Colloid gold particles with diameters of 40 nm were used as red-colored antibody labels for the visual detection of the antigen. The new dipstick sensor is capable of detecting down to 5 μg•l-1 (ppb; total inversion of the color signal) or 1 ppb (observation of color grading) of microcystin-LR. The course of the labeling reaction was observed via spectrometric wave shifts caused by the change of particle size during the binding of antibodies. Different stabilizing reagents showed that especially bovine serum albumin (BSA) and casein increase the assays sensitivity and the conjugate stability. Performance of the dipsticks was quantified by pattern processing of capture zone CCD images. Storage stability of dipsticks and conjugate suspensions over 115 days under different conditions were monitored. The ready-to-use dipsticks were successfully tested with microcystin-LR-spiked samples of outdoor drinking- and salt water and applied to the tissue of microcystin-fed mussels.",
keywords = "Dipstick, Gold nanoparticles, Immunochromatographic, Lateral flow, Microcystin, Toxin",
author = "Nils Tippk{\"o}tter and Henning St{\"u}ckmann and Stephen Kroll and Gunda Winkelmann and Udo Noack and Thomas Scheper and Roland Ulber",
year = "2009",
month = mar,
day = "24",
doi = "10.1007/s00216-009-2750-8",
language = "English",
volume = "394",
pages = "863--869",
journal = "Analytical and Bioanalytical Chemistry",
issn = "1618-2642",
publisher = "Springer Verlag",
number = "3",
}