A semi-quantitative dipstick assay for microcystin

verfasst von

Nils Tippkötter, Henning Stückmann, Stephen Kroll, Gunda Winkelmann, Udo Noack, Thomas Scheper, Roland Ulber

Abstract

An immunochromatographic lateral flow dipstick assay for the fast detection of microcystin-LR was developed. Colloid gold particles with diameters of 40 nm were used as red-colored antibody labels for the visual detection of the antigen. The new dipstick sensor is capable of detecting down to 5 μg•l^-1 (ppb; total inversion of the color signal) or 1 ppb (observation of color grading) of microcystin-LR. The course of the labeling reaction was observed via spectrometric wave shifts caused by the change of particle size during the binding of antibodies. Different stabilizing reagents showed that especially bovine serum albumin (BSA) and casein increase the assays sensitivity and the conjugate stability. Performance of the dipsticks was quantified by pattern processing of capture zone CCD images. Storage stability of dipsticks and conjugate suspensions over 115 days under different conditions were monitored. The ready-to-use dipsticks were successfully tested with microcystin-LR-spiked samples of outdoor drinking- and salt water and applied to the tissue of microcystin-fed mussels.

Organisationseinheit(en)

Institut für Technische Chemie

Externe Organisation(en)

Technische Universität Kaiserslautern
Noack Laboratorien GmbH

Typ

Artikel

Journal

Analytical and Bioanalytical Chemistry

Band

394

Seiten

863-869

Anzahl der Seiten

7

ISSN

1618-2642

Publikationsdatum

24.03.2009

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Analytische Chemie, Biochemie

Elektronische Version(en)

https://doi.org/10.1007/s00216-009-2750-8 (Zugang: Unbekannt)