Reversible coupling of glucoenzymes on fluoride-sensitive FET biosensors based on lectin-glucoprotein binding
- authored by
- R. Köneke, C. Menzel, R. Ulber, K. Schügerl, T. Scheper, M. Saleemuddin
- Abstract
New reloadable biosensors were developed by affinity binding of glucoenzymes peroxidase and glucose oxidase on pF-sensitive field-effect transistors (Si/SiO2/Si3N4/LaF3 layers). The basic measuring principle of these ion sensitive field-effect transistors is the current change depending on the concentration of F- ions. The immobilized glucoenzymes can be removed from the enzymatically inactive Concanavalin A basic membrane and fresh enzymes can be bound again. The principle of this reloadable biosensor is described in detail. Additionally, the biosensor was successfully integrated in a flow-injection analysis system for measuring glucose during a cultivation process.
- Organisation(s)
-
Institute of Technical Chemistry
- External Organisation(s)
-
Aligarh Muslim University
- Type
- Article
- Journal
- Biosensors and Bioelectronics
- Volume
- 11
- Pages
- 1229-1236
- No. of pages
- 8
- ISSN
- 0956-5663
- Publication date
- 1996
- Publication status
- Published
- Peer reviewed
- Yes
- ASJC Scopus subject areas
- Biotechnology, Biophysics, Biomedical Engineering, Electrochemistry
- Electronic version(s)
-
https://doi.org/10.1016/0956-5663(96)88088-2 (Access:
Unknown)
