Reversible coupling of glucoenzymes on fluoride-sensitive FET biosensors based on lectin-glucoprotein binding
- verfasst von
- R. Köneke, C. Menzel, R. Ulber, K. Schügerl, T. Scheper, M. Saleemuddin
- Abstract
New reloadable biosensors were developed by affinity binding of glucoenzymes peroxidase and glucose oxidase on pF-sensitive field-effect transistors (Si/SiO2/Si3N4/LaF3 layers). The basic measuring principle of these ion sensitive field-effect transistors is the current change depending on the concentration of F- ions. The immobilized glucoenzymes can be removed from the enzymatically inactive Concanavalin A basic membrane and fresh enzymes can be bound again. The principle of this reloadable biosensor is described in detail. Additionally, the biosensor was successfully integrated in a flow-injection analysis system for measuring glucose during a cultivation process.
- Organisationseinheit(en)
-
Institut für Technische Chemie
- Externe Organisation(en)
-
Aligarh Muslim University
- Typ
- Artikel
- Journal
- Biosensors and Bioelectronics
- Band
- 11
- Seiten
- 1229-1236
- Anzahl der Seiten
- 8
- ISSN
- 0956-5663
- Publikationsdatum
- 1996
- Publikationsstatus
- Veröffentlicht
- Peer-reviewed
- Ja
- ASJC Scopus Sachgebiete
- Biotechnologie, Biophysik, Biomedizintechnik, Elektrochemie
- Elektronische Version(en)
-
https://doi.org/10.1016/0956-5663(96)88088-2 (Zugang:
Unbekannt)
