Application of a flow injection fibre optic biosensor for the analysis of different amino acids
- authored by
- S. J. Lee, Th Scheper, A. F. Bückmann
- Abstract
The analysis of different amino acids is described using a fibre optic biosensor previously described in its principles. This biosensor works according to the measurement principle of flow injection analysis (FIA) and consists of a fluorosensor and a measurement cell operating under pressure. In the measurement cell, enzymes and molecular weight enlarged coenzyme PEG (MW 20000)-N6-(2-aminoethyl)-NAD(H) are confined behind a solid ultrafiltration membrane. Assays for the analysis of L-phenylalanine and L-alanine were developed. The analysis frequency is in the range of 1 to 2 samples per hour, and the sensor stability was found to be sensitive to the stability of each enzyme system used. This effect was studied in detail. The L-alanine assay was found to be especially reliable, sensitive, specific, and highly selective for the L-enantiomer.
- External Organisation(s)
-
University of Münster
Helmholtz Centre for Infection Research (HZI)
- Type
- Article
- Journal
- Biosensors and Bioelectronics
- Volume
- 9
- Pages
- 29-32
- No. of pages
- 4
- ISSN
- 0956-5663
- Publication date
- 1994
- Publication status
- Published
- Peer reviewed
- Yes
- ASJC Scopus subject areas
- Biotechnology, Biophysics, Biomedical Engineering, Electrochemistry
- Electronic version(s)
-
https://doi.org/10.1016/0956-5663(94)80012-X (Access:
Unknown)
