Application of a flow injection fibre optic biosensor for the analysis of different amino acids

verfasst von

S. J. Lee, Th Scheper, A. F. Bückmann

Abstract

The analysis of different amino acids is described using a fibre optic biosensor previously described in its principles. This biosensor works according to the measurement principle of flow injection analysis (FIA) and consists of a fluorosensor and a measurement cell operating under pressure. In the measurement cell, enzymes and molecular weight enlarged coenzyme PEG (MW 20000)-N⁶-(2-aminoethyl)-NAD(H) are confined behind a solid ultrafiltration membrane. Assays for the analysis of L-phenylalanine and L-alanine were developed. The analysis frequency is in the range of 1 to 2 samples per hour, and the sensor stability was found to be sensitive to the stability of each enzyme system used. This effect was studied in detail. The L-alanine assay was found to be especially reliable, sensitive, specific, and highly selective for the L-enantiomer.

Externe Organisation(en)

Westfälische Wilhelms-Universität Münster (WWU)
Helmholtz-Zentrum für Infektionsforschung GmbH (HZI)

Typ

Artikel

Journal

Biosensors and Bioelectronics

Band

9

Seiten

29-32

Anzahl der Seiten

4

ISSN

0956-5663

Publikationsdatum

1994

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Biotechnologie, Biophysik, Biomedizintechnik, Elektrochemie

Elektronische Version(en)

https://doi.org/10.1016/0956-5663(94)80012-X (Zugang: Unbekannt)