Fluorometric measurement of poly-β hydroxybutyrate in Alcaligenes eutrophus by flow cytometry and spectrofluorometry

authored by

A. Degelau, T. Scheper, J. E. Bailey, C. Guske

Abstract

Two fluorometric assays for the determination of poly-β-hydroxybutyrate (PHB) inside intact cells are presented in this paper, a spectrofluorometric method and one based on a laser flow cytometer. The principles of these assays are given, and the results of these methods are compared with those of a gas chromatographic assay. All assays were used for the monitoring of batch cultivations of Alcaligenes eutrophus. The correlation between all methods was very good; however, the fluorometric analysis was the fastest while the cytometric assay gave a direct insight into the PHB distribution over the population.

External Organisation(s)

ETH Zurich
Kelco Company
University of Münster

Type

Article

Journal

Applied Microbiology and Biotechnology

Volume

42

Pages

653-657

No. of pages

5

ISSN

0175-7598

Publication date

01.1995

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Biotechnology, Applied Microbiology and Biotechnology

Electronic version(s)

https://doi.org/10.1007/BF00171939 (Access: Unknown)