Fluorometric measurement of poly-β hydroxybutyrate in Alcaligenes eutrophus by flow cytometry and spectrofluorometry

verfasst von

A. Degelau, T. Scheper, J. E. Bailey, C. Guske

Abstract

Two fluorometric assays for the determination of poly-β-hydroxybutyrate (PHB) inside intact cells are presented in this paper, a spectrofluorometric method and one based on a laser flow cytometer. The principles of these assays are given, and the results of these methods are compared with those of a gas chromatographic assay. All assays were used for the monitoring of batch cultivations of Alcaligenes eutrophus. The correlation between all methods was very good; however, the fluorometric analysis was the fastest while the cytometric assay gave a direct insight into the PHB distribution over the population.

Externe Organisation(en)

ETH Zürich
Kelco Company
Westfälische Wilhelms-Universität Münster (WWU)

Typ

Artikel

Journal

Applied Microbiology and Biotechnology

Band

42

Seiten

653-657

Anzahl der Seiten

5

ISSN

0175-7598

Publikationsdatum

01.1995

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Biotechnologie, Angewandte Mikrobiologie und Biotechnologie

Elektronische Version(en)

https://doi.org/10.1007/BF00171939 (Zugang: Unbekannt)