Considerations in the Use of an Infinite-Dose System for Studying Surfactant Effects on Diffusion in Isolated Cuticles

authored by: Moritz Knoche, Martin J. Bukovac
Abstract: The effect of Triton X-100 [TX-100, α-[4-(1,1,3,3-tetramethylbutyl)phenyl]-ω-hydroxypoly(oxy-1,2-ethanediyl)] at concentrations below and above the critical micelle concentration (cmc, cmc = 0.019% w/w) on diffusion of 2-(1-[¹⁴C]naphthyl)acetic acid (NAA, 1 μM) through isolated tomato (Lycopersicon esculentum Mill. cv. Pik Red) fruit cuticular (CM) and dewaxed cuticular membranes (DCM) was studied using an infinite-dose system. TX-100 significantly increased time lags of NAA diffusion from 5 h (NAA only) and 5.8 h (0.01% w/v TX-100) to 7.4 h (0.1% TX-100) and 7.1 h (1% TX-100) when averaged over CM and DCM. Diffusion time lags in the absence and presence of TX-100 were greater for CM (6.1 h) than for DCM (5.2 h). In CM, 0.01% TX-100 increased NAA flux, while 0.1% had no effect and 1% TX-100 decreased the flux. In DCM, both 0.01 and 0.1% TX-100 enhanced the rate of NAA penetration, while flux was reduced in the presence of 1 % TX-100. Two different approaches for calculating partition and diffusion coefficients are presented, and their suitability for analyzing effects of spray additives on cuticular penetration of an active ingredient is discussed using the example of TX-100 effects on NAA penetration.
External Organisation(s): Michigan State University (MSU)
Type: Article
Journal: Journal of Agricultural and Food Chemistry
Volume: 42
Pages: 1013-1018
No. of pages: 6
ISSN: 0021-8561
Publication date: 01.04.1994
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: General Chemistry, General Agricultural and Biological Sciences
Electronic version(s): https://doi.org/10.1021/jf00040a034 (Access: Closed)

BibTeX

@article{2bfaca6cd39644c2bacd9a171c03b2b0,
title = "Considerations in the Use of an Infinite-Dose System for Studying Surfactant Effects on Diffusion in Isolated Cuticles",
abstract = "The effect of Triton X-100 [TX-100, α-[4-(1,1,3,3-tetramethylbutyl)phenyl]-ω-hydroxypoly(oxy-1,2-ethanediyl)] at concentrations below and above the critical micelle concentration (cmc, cmc = 0.019% w/w) on diffusion of 2-(1-[14C]naphthyl)acetic acid (NAA, 1 μM) through isolated tomato (Lycopersicon esculentum Mill. cv. Pik Red) fruit cuticular (CM) and dewaxed cuticular membranes (DCM) was studied using an infinite-dose system. TX-100 significantly increased time lags of NAA diffusion from 5 h (NAA only) and 5.8 h (0.01% w/v TX-100) to 7.4 h (0.1% TX-100) and 7.1 h (1% TX-100) when averaged over CM and DCM. Diffusion time lags in the absence and presence of TX-100 were greater for CM (6.1 h) than for DCM (5.2 h). In CM, 0.01% TX-100 increased NAA flux, while 0.1% had no effect and 1% TX-100 decreased the flux. In DCM, both 0.01 and 0.1% TX-100 enhanced the rate of NAA penetration, while flux was reduced in the presence of 1 % TX-100. Two different approaches for calculating partition and diffusion coefficients are presented, and their suitability for analyzing effects of spray additives on cuticular penetration of an active ingredient is discussed using the example of TX-100 effects on NAA penetration.",
author = "Moritz Knoche and Bukovac, {Martin J.}",
year = "1994",
month = apr,
day = "1",
doi = "10.1021/jf00040a034",
language = "English",
volume = "42",
pages = "1013--1018",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "4",
}