Considerations in the Use of an Infinite-Dose System for Studying Surfactant Effects on Diffusion in Isolated Cuticles

verfasst von: Moritz Knoche, Martin J. Bukovac
Abstract: The effect of Triton X-100 [TX-100, α-[4-(1,1,3,3-tetramethylbutyl)phenyl]-ω-hydroxypoly(oxy-1,2-ethanediyl)] at concentrations below and above the critical micelle concentration (cmc, cmc = 0.019% w/w) on diffusion of 2-(1-[¹⁴C]naphthyl)acetic acid (NAA, 1 μM) through isolated tomato (Lycopersicon esculentum Mill. cv. Pik Red) fruit cuticular (CM) and dewaxed cuticular membranes (DCM) was studied using an infinite-dose system. TX-100 significantly increased time lags of NAA diffusion from 5 h (NAA only) and 5.8 h (0.01% w/v TX-100) to 7.4 h (0.1% TX-100) and 7.1 h (1% TX-100) when averaged over CM and DCM. Diffusion time lags in the absence and presence of TX-100 were greater for CM (6.1 h) than for DCM (5.2 h). In CM, 0.01% TX-100 increased NAA flux, while 0.1% had no effect and 1% TX-100 decreased the flux. In DCM, both 0.01 and 0.1% TX-100 enhanced the rate of NAA penetration, while flux was reduced in the presence of 1 % TX-100. Two different approaches for calculating partition and diffusion coefficients are presented, and their suitability for analyzing effects of spray additives on cuticular penetration of an active ingredient is discussed using the example of TX-100 effects on NAA penetration.
Externe Organisation(en): Michigan State University (MSU)
Typ: Artikel
Journal: Journal of Agricultural and Food Chemistry
Band: 42
Seiten: 1013-1018
Anzahl der Seiten: 6
ISSN: 0021-8561
Publikationsdatum: 01.04.1994
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
ASJC Scopus Sachgebiete: Allgemeine Chemie, Allgemeine Agrar- und Biowissenschaften
Elektronische Version(en): https://doi.org/10.1021/jf00040a034 (Zugang: Geschlossen)

BibTeX

@article{2bfaca6cd39644c2bacd9a171c03b2b0,
title = "Considerations in the Use of an Infinite-Dose System for Studying Surfactant Effects on Diffusion in Isolated Cuticles",
abstract = "The effect of Triton X-100 [TX-100, α-[4-(1,1,3,3-tetramethylbutyl)phenyl]-ω-hydroxypoly(oxy-1,2-ethanediyl)] at concentrations below and above the critical micelle concentration (cmc, cmc = 0.019% w/w) on diffusion of 2-(1-[14C]naphthyl)acetic acid (NAA, 1 μM) through isolated tomato (Lycopersicon esculentum Mill. cv. Pik Red) fruit cuticular (CM) and dewaxed cuticular membranes (DCM) was studied using an infinite-dose system. TX-100 significantly increased time lags of NAA diffusion from 5 h (NAA only) and 5.8 h (0.01% w/v TX-100) to 7.4 h (0.1% TX-100) and 7.1 h (1% TX-100) when averaged over CM and DCM. Diffusion time lags in the absence and presence of TX-100 were greater for CM (6.1 h) than for DCM (5.2 h). In CM, 0.01% TX-100 increased NAA flux, while 0.1% had no effect and 1% TX-100 decreased the flux. In DCM, both 0.01 and 0.1% TX-100 enhanced the rate of NAA penetration, while flux was reduced in the presence of 1 % TX-100. Two different approaches for calculating partition and diffusion coefficients are presented, and their suitability for analyzing effects of spray additives on cuticular penetration of an active ingredient is discussed using the example of TX-100 effects on NAA penetration.",
author = "Moritz Knoche and Bukovac, {Martin J.}",
year = "1994",
month = apr,
day = "1",
doi = "10.1021/jf00040a034",
language = "English",
volume = "42",
pages = "1013--1018",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "4",
}