On-line analysis of fermentation media

authored by: W. J. Schmidt, H. D. Meyer, K. Schügerl, W. Kuhlmann, K. H. Bellgardt
Abstract: A continuous on-line analysis system for components of the medium during the anaerobic and aerobic fermentation of glucose by Saccharomyces cerevisiae is described. The procedures used include: analysis of the outlet gas by conventional analyzers (CO₂, O₂) and a mass spectrometer; continuous sampling of the liquid phase with microfiltration and subsequent determination of phosphate and glucose (spectrophotometer), glucose (polarimeter) and ammonium ion (ion-sensitive sensor); and continuous separation of volatile components and dissolved gases with diffusion through a silicone rubber membrane in high vacuum and determination of mass (m/z=18, 28, 31, 32, 40, 44) by means of a mass spectrometer. The system was tested on a batch reactor and a continuously operated three-stage cascade consisting of stirred tank reactors. This equipment was connected to a process computer. Glucose was quantified either polarimetrically or spectrophotometrically with p-hydroxybenzoic acid hydrazide; the latter was far more sensitive and was preferred to enzymatic methods, with which correlation was good. Only acetoin was found to interfere. Problems with operating a mass spectrometer for fermentation control in the exit stream and with membrane sampling are discussed.
External Organisation(s): Aesculap AG
Helmholtz Centre for Infection Research (HZI)
Type: Article
Journal: Analytica chimica acta
Volume: 163
Pages: 101-109
No. of pages: 9
ISSN: 0003-2670
Publication date: 1984
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Analytical Chemistry, Biochemistry, Environmental Chemistry, Spectroscopy
Electronic version(s): https://doi.org/10.1016/S0003-2670(00)81498-1 (Access: Closed)

BibTeX

@article{e4576a5d3e3a45b487af76d61bdffa70,
title = "On-line analysis of fermentation media",
abstract = "A continuous on-line analysis system for components of the medium during the anaerobic and aerobic fermentation of glucose by Saccharomyces cerevisiae is described. The procedures used include: analysis of the outlet gas by conventional analyzers (CO2, O2) and a mass spectrometer; continuous sampling of the liquid phase with microfiltration and subsequent determination of phosphate and glucose (spectrophotometer), glucose (polarimeter) and ammonium ion (ion-sensitive sensor); and continuous separation of volatile components and dissolved gases with diffusion through a silicone rubber membrane in high vacuum and determination of mass (m/z=18, 28, 31, 32, 40, 44) by means of a mass spectrometer. The system was tested on a batch reactor and a continuously operated three-stage cascade consisting of stirred tank reactors. This equipment was connected to a process computer. Glucose was quantified either polarimetrically or spectrophotometrically with p-hydroxybenzoic acid hydrazide; the latter was far more sensitive and was preferred to enzymatic methods, with which correlation was good. Only acetoin was found to interfere. Problems with operating a mass spectrometer for fermentation control in the exit stream and with membrane sampling are discussed.",
author = "Schmidt, {W. J.} and Meyer, {H. D.} and K. Sch{\"u}gerl and W. Kuhlmann and Bellgardt, {K. H.}",
year = "1984",
doi = "10.1016/S0003-2670(00)81498-1",
language = "English",
volume = "163",
pages = "101--109",
journal = "Analytica chimica acta",
issn = "0003-2670",
publisher = "Elsevier",
number = "C",
}