Application of Scanning Fluorometry for Monitoring of a Fermentation Process

authored by

B. Tartakovsky, M. Sheintuch, J. M. Hilmer, T. Scheper

Abstract

Conventional observation of a fermentation process by using NAD(P)H-dependent fluorescence provides a mean for cell monitoring, but its practical application has been very limited due to many factors that affect culture fluorescence. In this work we studied correlation of scanning fluorometry signals with various process variables and with cell metabolic states and showed that scanning fluorometry is more informative than conventional NAD(P)H-dependent fluorescence. A stepwise multiple-regression procedure has been used to select meaningful signals from the whole spectrum, and we found that with three or four components of the excitation-emission plane the process variables can be portrayed with a rather good accuracy. The approach is demonstrated on the examples of wild-type or recombinant Escherichia coli and Saccharomyces cerevisiae fermentations.

Organisation(s)

Institute of Technical Chemistry

External Organisation(s)

Technion-Israel Institute of Technology

Type

Article

Journal

Biotechnology progress

Volume

12

Pages

126-131

No. of pages

6

ISSN

8756-7938

Publication date

1996

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Biotechnology

Electronic version(s)

https://doi.org/10.1021/bp950045h (Access: Unknown)