Application of enzyme field-effect transistor sensor arrays as detectors in a flow-injection system for simultaneous monitoring of medium components. Part I. Preparation and calibration.

authored by: T. Kullick, M. Beyer, J. Henning, T. Lerch, R. Quack, A. Zeitz, B. Hitzmann, T. Scheper, K. Schürgerl
Abstract: Enzymes were (co)immobilized on the pH-sensitive gates of an eight-channel field-effect transistor (FET). Glucose was analysed with a glucose dehydrogenase (GDH) FET, maltose with a coimmobilized maltase (MAL)/GDH FET, sucrose with a coimmobilized invertase (INV)/GDH FET, lactose with a β-galactosidase (β-GAL)/galactose dehydrogenase (GALDH)-fusion protein FET and ethanol with a coimmobilized alcohol dehydrogenase (ADH)/aldehyde dehyrogenase (ALDH) FET. These EnFETs were integrated in FIA systems, calibrated and characterized with respect to buffer capacity, pH value, stirrer speed and NAD concentration and cross sensitivity. On account of the pH and buffer capacity sensitivity of the signals of the EnFETs, the pH value was monitored with a pH-FET of the array and the buffer capacity and substrate concentration were calculated from the shape of the signal of the FIA system. The EnFETs were stored at 4°C for many months without activity loss. They have satisfactory activity for performing a large number of analyses.
External Organisation(s): University of Münster
Type: Article
Journal: Analytica chimica acta
Volume: 296
Pages: 263-269
No. of pages: 7
ISSN: 0003-2670
Publication date: 20.10.1994
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Analytical Chemistry, Biochemistry, Environmental Chemistry, Spectroscopy
Electronic version(s): https://doi.org/10.1016/0003-2670(94)80245-9 (Access: Unknown)

BibTeX

@article{dce8a71e5240491594df4840aadc9555,
title = "Application of enzyme field-effect transistor sensor arrays as detectors in a flow-injection system for simultaneous monitoring of medium components. Part I. Preparation and calibration.",
abstract = "Enzymes were (co)immobilized on the pH-sensitive gates of an eight-channel field-effect transistor (FET). Glucose was analysed with a glucose dehydrogenase (GDH) FET, maltose with a coimmobilized maltase (MAL)/GDH FET, sucrose with a coimmobilized invertase (INV)/GDH FET, lactose with a β-galactosidase (β-GAL)/galactose dehydrogenase (GALDH)-fusion protein FET and ethanol with a coimmobilized alcohol dehydrogenase (ADH)/aldehyde dehyrogenase (ALDH) FET. These EnFETs were integrated in FIA systems, calibrated and characterized with respect to buffer capacity, pH value, stirrer speed and NAD concentration and cross sensitivity. On account of the pH and buffer capacity sensitivity of the signals of the EnFETs, the pH value was monitored with a pH-FET of the array and the buffer capacity and substrate concentration were calculated from the shape of the signal of the FIA system. The EnFETs were stored at 4°C for many months without activity loss. They have satisfactory activity for performing a large number of analyses.",
keywords = "EnFET, Enzymatic methods, Field effect transistors, Flow injection, Sensors",
author = "T. Kullick and M. Beyer and J. Henning and T. Lerch and R. Quack and A. Zeitz and B. Hitzmann and T. Scheper and K. Sch{\"u}rgerl",
note = "Funding information: &GAL/GALDH and thank the Ministry of Re-searcho f Technologyo f the Federal Republic of Germany,B onn, for the financial support.",
year = "1994",
month = oct,
day = "20",
doi = "10.1016/0003-2670(94)80245-9",
language = "English",
volume = "296",
pages = "263--269",
journal = "Analytica chimica acta",
issn = "0003-2670",
publisher = "Elsevier",
number = "3",
}