Movement of a barley stripe mosaic virus chimera with a tobacco mosaic virus movement protein

authored by: A. G. Solovyev, D. A. Zelenina, E. I. Savenkov, V. Z. Grdzelishvili, S. Yu Morozov, D. E. Lesemann, E. Maiss, R. Casper, J. G. Atabekov
Abstract: The tobacco mosaic virus (TMV) 30K movement protein (MP) gene was inserted into a full-length cDNA clone of barley stripe mosaic virus (BSMV) RNAβ replacing the triple gene block (TGB). The resulting recombinant ND-MPT genome, consisting of Infectious wt transcripts of BSMV RNAs α and γ, together with the hybrid RNAβ transcript, was inoculated onto test plants to study the functional compatibility between the BSMV TGB-adapted genetic system and the tobamovirus transport gene. ND-MPT infected the inoculated leaves of Nicotiana benthamiana and Chenopodium amaranticolor, which are common hosts for the parental viruses; the size, growth rate, and morphology of local lesions on C. amaranticolor were influenced by the foreign MP gene. However, the hybrid virus failed to infect barley, N. tabacum (var. Samsun), and N. clevelandii, the selective hosts. Thus, the TMV MP was able to functionally substitute for the BSMV TGB-coded MPs, i.e., the 30K MP functioned independently of any other BSMV sequences. However, the TMV MP gene promoted the cell-to-cell movement in a host-dependent manner.
External Organisation(s): Biologische Bundesanstalt für Land- und Forstwirtschaft (BBA)
Lomonosov Moscow State University
Type: Article
Journal: VIROLOGY
Volume: 217
Pages: 435-441
No. of pages: 7
ISSN: 0042-6822
Publication date: 15.03.1996
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Virology
Electronic version(s): https://doi.org/10.1006/viro.1996.0137 (Access: Open)

BibTeX

@article{d62d69fd5576461e8cfe6b23f9edd2b0,
title = "Movement of a barley stripe mosaic virus chimera with a tobacco mosaic virus movement protein",
abstract = "The tobacco mosaic virus (TMV) 30K movement protein (MP) gene was inserted into a full-length cDNA clone of barley stripe mosaic virus (BSMV) RNAβ replacing the triple gene block (TGB). The resulting recombinant ND-MPT genome, consisting of Infectious wt transcripts of BSMV RNAs α and γ, together with the hybrid RNAβ transcript, was inoculated onto test plants to study the functional compatibility between the BSMV TGB-adapted genetic system and the tobamovirus transport gene. ND-MPT infected the inoculated leaves of Nicotiana benthamiana and Chenopodium amaranticolor, which are common hosts for the parental viruses; the size, growth rate, and morphology of local lesions on C. amaranticolor were influenced by the foreign MP gene. However, the hybrid virus failed to infect barley, N. tabacum (var. Samsun), and N. clevelandii, the selective hosts. Thus, the TMV MP was able to functionally substitute for the BSMV TGB-coded MPs, i.e., the 30K MP functioned independently of any other BSMV sequences. However, the TMV MP gene promoted the cell-to-cell movement in a host-dependent manner.",
author = "Solovyev, {A. G.} and Zelenina, {D. A.} and Savenkov, {E. I.} and Grdzelishvili, {V. Z.} and Morozov, {S. Yu} and Lesemann, {D. E.} and E. Maiss and R. Casper and Atabekov, {J. G.}",
year = "1996",
month = mar,
day = "15",
doi = "10.1006/viro.1996.0137",
language = "English",
volume = "217",
pages = "435--441",
journal = "VIROLOGY",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",
}