Efficient generation of 2E-hexenal by a hydroperoxide lyase from mung bean seedlings

authored by: B. Rehbock, D. Ganßer, R. G. Berger
Abstract: 2E-hexenal was generated with a high molar conversion rate by the incubation of a hydroperoxide lyase containing extract from mung bean seedlings and its substrate, 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT). Various parameters affected the yield of 2E-hexenal and the conversion rate: hydroperoxide lyase activity was especially, pronounced in seedlings older than 10 days. The cleavage of 13-HPOT by a solubilized enzyme extract proceeded best at pH 6.5. Time-course studies showed that the majority of flavour compounds was already generated during the first 10 min of the incubation period. Using optimized reaction conditions, a maximum yield of 1062 mg kg^-1 2E-hexenal was obtained with 32.6 mmol kg^-1 (10.1 gkg^-1) 13-HPOT. However, the conversion rate was highest (maximum 86.3%) at a low substrate concentration, indicating a suicidal behaviour of the hydroperoxide lyase. Enzymatically hydrolyzed and dioxygenated linseed oil was a suitable alternative to pure linolenic acid.
Organisation(s): Institute of Food Chemistry
Type: Article
Journal: Food chemistry
Volume: 63
Pages: 161-165
No. of pages: 5
ISSN: 0308-8146
Publication date: 30.10.1998
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Analytical Chemistry, Food Science
Sustainable Development Goals: SDG 3 - Good Health and Well-being
Electronic version(s): https://doi.org/10.1016/S0308-8146(98)00042-9 (Access: Unknown)

BibTeX

@article{b2c12e6863064684aaf3d0f849942791,
title = "Efficient generation of 2E-hexenal by a hydroperoxide lyase from mung bean seedlings",
abstract = "2E-hexenal was generated with a high molar conversion rate by the incubation of a hydroperoxide lyase containing extract from mung bean seedlings and its substrate, 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT). Various parameters affected the yield of 2E-hexenal and the conversion rate: hydroperoxide lyase activity was especially, pronounced in seedlings older than 10 days. The cleavage of 13-HPOT by a solubilized enzyme extract proceeded best at pH 6.5. Time-course studies showed that the majority of flavour compounds was already generated during the first 10 min of the incubation period. Using optimized reaction conditions, a maximum yield of 1062 mg kg-1 2E-hexenal was obtained with 32.6 mmol kg-1 (10.1 gkg-1) 13-HPOT. However, the conversion rate was highest (maximum 86.3%) at a low substrate concentration, indicating a suicidal behaviour of the hydroperoxide lyase. Enzymatically hydrolyzed and dioxygenated linseed oil was a suitable alternative to pure linolenic acid.",
author = "B. Rehbock and D. Gan{\ss}er and Berger, {R. G.}",
note = "Funding information: This work was supported by the EU project AIR3-CT94-2060a nd by the Fonds der ChemischenIn dustrie, Frankfurt.",
year = "1998",
month = oct,
day = "30",
doi = "10.1016/S0308-8146(98)00042-9",
language = "English",
volume = "63",
pages = "161--165",
journal = "Food chemistry",
issn = "0308-8146",
publisher = "Elsevier Ltd.",
number = "2",
}