Free Bioverit® II implants coated with a nanoporous silica layer in a mouse ear model

A histological study

authored by: Julia C. Vogt, Gudrun Brandes, Nina Ehlert, Peter Behrens, Ingo Nolte, Peter P. Mueller, Thomas Lenarz, Martin Stieve
Abstract: The objective of this study is to evaluate the suitability of a mouse middle ear model for testing ossicular replacement materials. Twenty-four BALB/c mice are implanted with the bioglass-ceramic Bioverit® II which is coated with a silica-nanostructure or with plain Bioverit® II as a control. After 2, 6, and 12 weeks, 4 mice per group are sacrificed and both complete petrous bones are analyzed histologically. All implants revealed in situ an incipient growth of thin connective tissue layers over the surface, followed by a spreading of epithelial cells. The osseogenic response which is increasing with time is more intense in the coated Bioverit® II specimens. The absence of inflammatory cells suggests an excellent biocompatibility of the silica nano structure. As the results are comparable to a study with the same materials in rabbits, the mouse model described is highly suitable for evaluation of new ossicular replacement materials. Additionally, by gene expression analysis a more detailed insight into cellular interactions of the middle ear is offered.
Organisation(s): Institute of Inorganic Chemistry
External Organisation(s): Hannover Medical School (MHH)
University of Veterinary Medicine of Hannover, Foundation
Helmholtz Centre for Infection Research (HZI)
Type: Article
Journal: Journal of Biomaterials Applications
Volume: 24
Pages: 175-191
No. of pages: 17
ISSN: 0885-3282
Publication date: 01.08.2009
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Biomaterials, Biomedical Engineering
Electronic version(s): https://doi.org/10.1177/0885328208095469 (Access: Unknown)

BibTeX

@article{9e9d1cf9fda84de7b04699a42a53d7c2,
title = "Free Bioverit{\textregistered} II implants coated with a nanoporous silica layer in a mouse ear model: A histological study",
abstract = "The objective of this study is to evaluate the suitability of a mouse middle ear model for testing ossicular replacement materials. Twenty-four BALB/c mice are implanted with the bioglass-ceramic Bioverit{\textregistered} II which is coated with a silica-nanostructure or with plain Bioverit{\textregistered} II as a control. After 2, 6, and 12 weeks, 4 mice per group are sacrificed and both complete petrous bones are analyzed histologically. All implants revealed in situ an incipient growth of thin connective tissue layers over the surface, followed by a spreading of epithelial cells. The osseogenic response which is increasing with time is more intense in the coated Bioverit{\textregistered} II specimens. The absence of inflammatory cells suggests an excellent biocompatibility of the silica nano structure. As the results are comparable to a study with the same materials in rabbits, the mouse model described is highly suitable for evaluation of new ossicular replacement materials. Additionally, by gene expression analysis a more detailed insight into cellular interactions of the middle ear is offered.",
keywords = "Biocompatibility, Bioverit{\textregistered} II, Histology, Middle ear, Mouse, Silica-nanocoating",
author = "Vogt, {Julia C.} and Gudrun Brandes and Nina Ehlert and Peter Behrens and Ingo Nolte and Mueller, {Peter P.} and Thomas Lenarz and Martin Stieve",
year = "2009",
month = aug,
day = "1",
doi = "10.1177/0885328208095469",
language = "English",
volume = "24",
pages = "175--191",
journal = "Journal of Biomaterials Applications",
issn = "0885-3282",
publisher = "SAGE Publications Ltd",
number = "2",
}