Femtosecond Laser Fabricated Spike Structures for Selective Control of Cellular Behavior

authored by: Sabrina Schlie, Elena Fadeeva, Jürgen Koch, Anaclet Ngezahayo, Boris N. Chichkov
Abstract: In this study we investigate the potential of femtosecond laser generated micrometer sized spike structures as functional surfaces for selective cell controlling. The spike dimensions as well as the average spike to spike distance can be easily tuned by varying the process parameters. Moreover, negative replications in soft materials such as silicone elastomer can be produced. This allows tailoring of wetting properties of the spike structures and their negative replicas representing a reduced surface contact area. Furthermore, we investigated material effects on cellular behavior. By comparing human fibroblasts and SH-SY5Y neuroblastoma cells we found that the influence of the material was cell specific. The cells not only changed their morphology, but also the cell growth was affected. Whereas, neuroblastoma cells proliferated at the same rate on the spike structures as on the control surfaces, the proliferation of fibroblasts was reduced by the spike structures. These effects can result from the cell specific adhesion patterns as shown in this work. These findings show a possibility to design defined surface microstructures, which could control cellular behavior in a cell specific manner.
Organisation(s): Institute of Cell Biology and Biophysics
External Organisation(s): Laser Zentrum Hannover e.V. (LZH)
Type: Article
Journal: Journal of Biomaterials Applications
Volume: 25
Pages: 217-233
No. of pages: 17
ISSN: 0885-3282
Publication date: 22.09.2009
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Biomaterials, Biomedical Engineering
Electronic version(s): https://doi.org/10.1177/0885328209345553 (Access: Restricted)

BibTeX

@article{8fd32d7b13384d1fba4214aa2597bbc6,
title = "Femtosecond Laser Fabricated Spike Structures for Selective Control of Cellular Behavior",
abstract = "In this study we investigate the potential of femtosecond laser generated micrometer sized spike structures as functional surfaces for selective cell controlling. The spike dimensions as well as the average spike to spike distance can be easily tuned by varying the process parameters. Moreover, negative replications in soft materials such as silicone elastomer can be produced. This allows tailoring of wetting properties of the spike structures and their negative replicas representing a reduced surface contact area. Furthermore, we investigated material effects on cellular behavior. By comparing human fibroblasts and SH-SY5Y neuroblastoma cells we found that the influence of the material was cell specific. The cells not only changed their morphology, but also the cell growth was affected. Whereas, neuroblastoma cells proliferated at the same rate on the spike structures as on the control surfaces, the proliferation of fibroblasts was reduced by the spike structures. These effects can result from the cell specific adhesion patterns as shown in this work. These findings show a possibility to design defined surface microstructures, which could control cellular behavior in a cell specific manner.",
keywords = "biomedicine, cellular adhesion, femtosecond laser, microstructures, surface topography, water contact angle",
author = "Sabrina Schlie and Elena Fadeeva and J{\"u}rgen Koch and Anaclet Ngezahayo and Chichkov, {Boris N.}",
year = "2009",
month = sep,
day = "22",
doi = "10.1177/0885328209345553",
language = "English",
volume = "25",
pages = "217--233",
journal = "Journal of Biomaterials Applications",
issn = "0885-3282",
publisher = "SAGE Publications Ltd",
number = "3",
}