On-line monitoring of enzyme-catalyzed biotransformations with biosensors

authored by
Frank Lammers, Thomas Scheper
Abstract

A flow injection analysis system for on-line monitoring of enzyme-catalyzed L-ornithine, L-methionine, and fructose synthesis is presented. An enzyme thermistor was used as detector. The enzyme-catalyzed reactions were performed in a simple batch reactor with immobilized biocatalysts (arginase, acylase, invertase, or glucose isomerase, respectively). The bioreactor was connected with a computer-controlled FIA system. The method allows continuous monitoring and optimization of an enzyme-catalyzed process.

Organisation(s)
Institute of Technical Chemistry
Type
Article
Journal
Enzyme and microbial technology
Volume
20
Pages
432-436
No. of pages
5
ISSN
0141-0229
Publication date
01.05.1997
Publication status
Published
Peer reviewed
Yes
ASJC Scopus subject areas
Biotechnology, Bioengineering, Biochemistry, Applied Microbiology and Biotechnology
Electronic version(s)
https://doi.org/10.1016/S0141-0229(96)00171-8 (Access: Unknown)