The production of 2,3-butanediol by fermentation of high test molasses

authored by: A. S. Afschar, K. H. Bellgardt, C. E. Vaz Rossell, A. Czok, K. Schaller
Abstract: Klebsiella oxytoca fermented 199 g·l^-1 high test or invert molasses using batch fermentation with substrate shift to produce 95.2-98.6 g 2,3-butanediol·l^-1 and 2,4-4.3 g acetoin·l^-1 with a diol yield of 96-100% of the theoretical value and a diol productivity of 1.0-1.1 g·l^-1·h^-1. Fermentation was performed numerous times with molasses in repeated batch culture with cell recovery. Such repeated batch fermentation, in addition to a high product yield, also showed a very high product concentration. For example, 118 g 2,3-butanediol·l^-1 and 2.3 g acetoin·l^-1 were produced from 280 g·l^-1 of high test molasses. The diol productivity in this fermentation amounted to 2.4 g·l^-1·h^-1 and can undoubtedly be further increased by increasing the cell concentration. Because the Klebsiella cultures ferment 2,3-butanediol at an extremely high rate once the sugar has been consumed, the culture was inhibited completely by the addition of 15 g ethanol·l^-1 and switching off aeration.
Organisation(s): Institute of Technical Chemistry
External Organisation(s): Helmholtz Centre for Infection Research (HZI)
Centro de Technologia Copersucar
Type: Article
Journal: Applied Microbiology and Biotechnology
Volume: 34
Pages: 582-585
No. of pages: 4
ISSN: 0175-7598
Publication date: 02.1991
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Biotechnology, Applied Microbiology and Biotechnology
Electronic version(s): https://doi.org/10.1007/BF00167903 (Access: Closed)

BibTeX

@article{2cbec068c0684562a0ebc6206eebe995,
title = "The production of 2,3-butanediol by fermentation of high test molasses",
abstract = "Klebsiella oxytoca fermented 199 g·l-1 high test or invert molasses using batch fermentation with substrate shift to produce 95.2-98.6 g 2,3-butanediol·l-1 and 2,4-4.3 g acetoin·l-1 with a diol yield of 96-100% of the theoretical value and a diol productivity of 1.0-1.1 g·l-1·h-1. Fermentation was performed numerous times with molasses in repeated batch culture with cell recovery. Such repeated batch fermentation, in addition to a high product yield, also showed a very high product concentration. For example, 118 g 2,3-butanediol·l-1 and 2.3 g acetoin·l-1 were produced from 280 g·l-1 of high test molasses. The diol productivity in this fermentation amounted to 2.4 g·l-1·h-1 and can undoubtedly be further increased by increasing the cell concentration. Because the Klebsiella cultures ferment 2,3-butanediol at an extremely high rate once the sugar has been consumed, the culture was inhibited completely by the addition of 15 g ethanol·l-1 and switching off aeration.",
author = "Afschar, {A. S.} and Bellgardt, {K. H.} and {Vaz Rossell}, {C. E.} and A. Czok and K. Schaller",
year = "1991",
month = feb,
doi = "10.1007/BF00167903",
language = "English",
volume = "34",
pages = "582--585",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "5",
}