Monitoring and control of biotechnological production processes by Bio-FET-FIA-sensors

authored by

U. Brand, L. Brandes, V. Koch, T. Kullik, B. Reinhardt, F. Rüther, T. Scheper, K. Schügerl, S. Wang, X. Wu, R. Ferretti, S. Prasad, D. Wilhelm

Abstract

Single and multisensor field effect transistors (FET) with a pH-sensitive Si/SiO₂/Si₃N₄/Ta₂O₅-gate and reference electrode (for single sensor) were developed and used for manufacturing the following biological (Bio)-FETs: for glucose analysis, glucose oxidase-FET (GOD-FET); for urea analysis, urease-FET; and for cephalosporin C analysis, cephalosporinase-FET. The GOD-FETs were integrated into flow injection analysis (FIA) of the Eppendorf variables analyser (EVA) system and used for monitoring the glucose concentration in microbial cultivation and production processes with recombinant Escherichia coli K12 MF, recombinant E. coli JM103, Saccharomyces cerevisiae H620, and Candida boidinii. Urease-FET-FIA was used to monitor the urea concentration in a simulated cultivation of Cephalosporium acremonium and urease-FET-FIA and GOD-FET-FIA for the monitoring of urea and glucose concentrations in simulated S. cerevisiae cultivations.

Organisation(s)

Institute of Technical Chemistry

Type

Article

Journal

Applied Microbiology and Biotechnology

Volume

36

Pages

167-172

No. of pages

6

ISSN

0175-7598

Publication date

11.1991

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Biotechnology, Applied Microbiology and Biotechnology

Electronic version(s)

https://doi.org/10.1007/BF00164414 (Access: Unknown)