Application of an enzyme thermistor for the determination of glucose in complex fermentation media

authored by: G. Wehnert, A. Sauerbrei, Th Bayer, Th Scheper, K. Schügerl, Th Herold
Abstract: An enzyme thermistor was used for on-line glucose determination during cultivation of Cephalosporium acremonium for a period of 160 h. The complex medium consisted of 100 g 1^-1 peanut meal. Automatic control and data registration were achieved by interfacing a process computer with the thermistor unit. Problem caused by long-term application are discussed. On-line and off-line data obtained with the enzyme thermistor are compared with the results obtained with a commercial glucose analyzer. The results of these experiments show that an enzyme thermistor can be used to determine glucose concentration in complex fermentation media under real cultivation conditions for about 60 h.
Organisation(s): Institute of Technical Chemistry
External Organisation(s): Sartorius AG
Type: Article
Journal: Analytica chimica acta
Volume: 200
Pages: 73-78
No. of pages: 6
ISSN: 0003-2670
Publication date: 1987
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Analytical Chemistry, Biochemistry, Environmental Chemistry, Spectroscopy
Electronic version(s): https://doi.org/10.1016/S0003-2670(00)83759-9 (Access: Unknown)