Application of an enzyme thermistor for the determination of glucose in complex fermentation media

verfasst von

G. Wehnert, A. Sauerbrei, Th Bayer, Th Scheper, K. Schügerl, Th Herold

Abstract

An enzyme thermistor was used for on-line glucose determination during cultivation of Cephalosporium acremonium for a period of 160 h. The complex medium consisted of 100 g 1^-1 peanut meal. Automatic control and data registration were achieved by interfacing a process computer with the thermistor unit. Problem caused by long-term application are discussed. On-line and off-line data obtained with the enzyme thermistor are compared with the results obtained with a commercial glucose analyzer. The results of these experiments show that an enzyme thermistor can be used to determine glucose concentration in complex fermentation media under real cultivation conditions for about 60 h.

Organisationseinheit(en)

Institut für Technische Chemie

Externe Organisation(en)

Sartorius AG

Typ

Artikel

Journal

Analytica chimica acta

Band

200

Seiten

73-78

Anzahl der Seiten

6

ISSN

0003-2670

Publikationsdatum

1987

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Analytische Chemie, Biochemie, Umweltchemie, Spektroskopie

Elektronische Version(en)

https://doi.org/10.1016/S0003-2670(00)83759-9 (Zugang: Unbekannt)