Continuous on-line monitoring of intracellular enzyme activity

authored by

N. Ahlmann, A. Niehoff, U. Rinas, Th Scheper, K. Schügerl

Abstract

For optimal cultivation of recombinant E. coli cells, it is necessary to monitor the intracellular enzyme activity by a fast, reliable and simple method. A modified air-segmented flow system is described for the on-line determination of intracellular penicillin-G-acylase (E.C. 3.5.1.11) during the cultivation of genetically modified E. coli 5K(pHM12). The results are compared to those obtained by different conventional off-line techniques.

Organisation(s)

Institute of Technical Chemistry

Type

Article

Journal

Analytica chimica acta

Volume

190

Pages

221-226

No. of pages

6

ISSN

0003-2670

Publication date

1986

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Analytical Chemistry, Biochemistry, Environmental Chemistry, Spectroscopy

Electronic version(s)

https://doi.org/10.1016/S0003-2670(00)82883-4 (Access: Unknown)