Continuous on-line monitoring of intracellular enzyme activity
- verfasst von
- N. Ahlmann, A. Niehoff, U. Rinas, Th Scheper, K. Schügerl
- Abstract
For optimal cultivation of recombinant E. coli cells, it is necessary to monitor the intracellular enzyme activity by a fast, reliable and simple method. A modified air-segmented flow system is described for the on-line determination of intracellular penicillin-G-acylase (E.C. 3.5.1.11) during the cultivation of genetically modified E. coli 5K(pHM12). The results are compared to those obtained by different conventional off-line techniques.
- Organisationseinheit(en)
-
Institut für Technische Chemie
- Typ
- Artikel
- Journal
- Analytica chimica acta
- Band
- 190
- Seiten
- 221-226
- Anzahl der Seiten
- 6
- ISSN
- 0003-2670
- Publikationsdatum
- 1986
- Publikationsstatus
- Veröffentlicht
- Peer-reviewed
- Ja
- ASJC Scopus Sachgebiete
- Analytische Chemie, Biochemie, Umweltchemie, Spektroskopie
- Elektronische Version(en)
-
https://doi.org/10.1016/S0003-2670(00)82883-4 (Zugang:
Unbekannt)
