On-line immunoanalysis for bioprocess control

authored by

C. Middendorf, B. Schulze, R. Freitag, Th Scheper, M. Howaldt, H. Hoffmann

Abstract

Immunoanalytical techniques such as ELISA are often used for the detection of proteins produced in cultivation processes. Owing to the difficulty of automating of the time-consuming traditional ELISA, there is an intense demand for a suitable on-line monitoring method. Combining well-known immunoassays with the FIA technique, we present the heterogeneous and the turbidimetric immuno-FIA methods. The following proteins were investigated with these FIA methods: thermostable pullulanase, IgG, antithrombin III, and recombinant tissue-type plasminogen activator. In the cases of pullulanase and monoclonal mouse IgG, the turbidimetric immuno-FIA was used for on-line analysis of the cultivation process. Results are presented here to demonstrate the effectiveness and application of these immunoanalyses.

External Organisation(s)

University of Münster
Boehringer Ingelheim

Type

Article

Journal

Journal of biotechnology

Volume

31

Pages

395-403

No. of pages

9

ISSN

0168-1656

Publication date

12.1993

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Biotechnology, Bioengineering, Applied Microbiology and Biotechnology

Electronic version(s)

https://doi.org/10.1016/0168-1656(93)90083-Y (Access: Unknown)