On-line immunoanalysis for bioprocess control

verfasst von

C. Middendorf, B. Schulze, R. Freitag, Th Scheper, M. Howaldt, H. Hoffmann

Abstract

Immunoanalytical techniques such as ELISA are often used for the detection of proteins produced in cultivation processes. Owing to the difficulty of automating of the time-consuming traditional ELISA, there is an intense demand for a suitable on-line monitoring method. Combining well-known immunoassays with the FIA technique, we present the heterogeneous and the turbidimetric immuno-FIA methods. The following proteins were investigated with these FIA methods: thermostable pullulanase, IgG, antithrombin III, and recombinant tissue-type plasminogen activator. In the cases of pullulanase and monoclonal mouse IgG, the turbidimetric immuno-FIA was used for on-line analysis of the cultivation process. Results are presented here to demonstrate the effectiveness and application of these immunoanalyses.

Externe Organisation(en)

Westfälische Wilhelms-Universität Münster (WWU)
Boehringer Ingelheim

Typ

Artikel

Journal

Journal of biotechnology

Band

31

Seiten

395-403

Anzahl der Seiten

9

ISSN

0168-1656

Publikationsdatum

12.1993

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Biotechnologie, Bioengineering, Angewandte Mikrobiologie und Biotechnologie

Elektronische Version(en)

https://doi.org/10.1016/0168-1656(93)90083-Y (Zugang: Unbekannt)