Two new fluorogenic substrates for the detection of Penicillin-G-acylase activity
- authored by
- Th Scheper, M. Weiss, K. Schügerl
- Abstract
Two fluorogenic substrates are described for the detection of Penicillin-G-acylase (EC 3.5.1.11) activity in coloured cell-containing media and inside single cells. A sensitive enzyme assay is based on the use of 7-phenylacetyl-4-alkylcoumarinylamides. Linear response is obtained in the range 20-300 μU m-1. After the cells have been made permeable, intracellular enzyme activity can be monitored. It is thus possible to label enzyme-containing cells and to distinguish them from enzyme-free cells by fluorescence microscopy or laser-flow cytometry.
- Organisation(s)
-
Institute of Technical Chemistry
- Type
- Article
- Journal
- Analytica chimica acta
- Volume
- 182
- Pages
- 203-206
- No. of pages
- 4
- ISSN
- 0003-2670
- Publication date
- 1986
- Publication status
- Published
- Peer reviewed
- Yes
- ASJC Scopus subject areas
- Analytical Chemistry, Biochemistry, Environmental Chemistry, Spectroscopy
- Electronic version(s)
-
https://doi.org/10.1016/S0003-2670(00)82451-4 (Access:
Unknown)
