Two new fluorogenic substrates for the detection of Penicillin-G-acylase activity

verfasst von

Th Scheper, M. Weiss, K. Schügerl

Abstract

Two fluorogenic substrates are described for the detection of Penicillin-G-acylase (EC 3.5.1.11) activity in coloured cell-containing media and inside single cells. A sensitive enzyme assay is based on the use of 7-phenylacetyl-4-alkylcoumarinylamides. Linear response is obtained in the range 20-300 μU m^-1. After the cells have been made permeable, intracellular enzyme activity can be monitored. It is thus possible to label enzyme-containing cells and to distinguish them from enzyme-free cells by fluorescence microscopy or laser-flow cytometry.

Organisationseinheit(en)

Institut für Technische Chemie

Typ

Artikel

Journal

Analytica chimica acta

Band

182

Seiten

203-206

Anzahl der Seiten

4

ISSN

0003-2670

Publikationsdatum

1986

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Analytische Chemie, Biochemie, Umweltchemie, Spektroskopie

Elektronische Version(en)

https://doi.org/10.1016/S0003-2670(00)82451-4 (Zugang: Unbekannt)