Statistical aspects of design and validation of microtitre-plate-based linear and non-linear parallel in vitro bioassays
- authored by
- Hanne Zimmermann, Daniel Gerhard, Theo Dingermann, Ludwig A. Hothorn
- Abstract
Assay validation was performed using four consecutive experiments with the related statistical evaluation. A cell-based assay on microtitre plates measured repeatedly within 1 day and on consecutive days was chosen as the model. The following problems were addressed: (i) choosing an appropriate design on a plate to avoid heterogeneities, (ii) quantification of all sources of variability and (iii) selecting between linear and non-linear parallel line assays. A mixed model was used with the random factors: rows, columns and plates and fixed effect factors with either linear or non-linear parallel line models.
- Organisation(s)
-
Department of Biostatistics
- External Organisation(s)
-
Roche Diagnostics GmbH
Goethe University Frankfurt
- Type
- Article
- Journal
- Biotechnology journal
- Volume
- 5
- Pages
- 62-74
- No. of pages
- 13
- ISSN
- 1860-6768
- Publication date
- 12.01.2010
- Publication status
- Published
- Peer reviewed
- Yes
- ASJC Scopus subject areas
- Applied Microbiology and Biotechnology, Molecular Medicine
- Electronic version(s)
-
https://doi.org/10.1002/biot.200900146 (Access:
Closed)
https://hal.science/hal-00527653/file/PEER_stage2_10.1002%252Fbiot.200900146.pdf (Access: Open)
