Statistical aspects of design and validation of microtitre-plate-based linear and non-linear parallel in vitro bioassays

verfasst von

Hanne Zimmermann, Daniel Gerhard, Theo Dingermann, Ludwig A. Hothorn

Abstract

Assay validation was performed using four consecutive experiments with the related statistical evaluation. A cell-based assay on microtitre plates measured repeatedly within 1 day and on consecutive days was chosen as the model. The following problems were addressed: (i) choosing an appropriate design on a plate to avoid heterogeneities, (ii) quantification of all sources of variability and (iii) selecting between linear and non-linear parallel line assays. A mixed model was used with the random factors: rows, columns and plates and fixed effect factors with either linear or non-linear parallel line models.

Organisationseinheit(en)

Abteilung Biostatistik

Externe Organisation(en)

Roche Diagnostics GmbH
Goethe-Universität Frankfurt am Main

Typ

Artikel

Journal

Biotechnology journal

Band

5

Seiten

62-74

Anzahl der Seiten

13

ISSN

1860-6768

Publikationsdatum

12.01.2010

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Angewandte Mikrobiologie und Biotechnologie, Molekularmedizin

Elektronische Version(en)

https://doi.org/10.1002/biot.200900146 (Zugang: Geschlossen)
https://hal.science/hal-00527653/file/PEER_stage2_10.1002%252Fbiot.200900146.pdf (Zugang: Offen)