Hitherto unknown [Fe-Fe]-hydrogenase gene diversity in anaerobes and anoxic enrichments from a moderately acidic fen

authored by

Oliver Schmidt, Harold L. Drake, Marcus A. Horn

Abstract

Newly designed primers for [Fe-Fe]-hydrogenases indicated that (i) fermenters, acetogens, and undefined species in a fen harbor hitherto unknown hydrogenases and (ii) Clostridium- and Thermosinus-related primary fermenters, as well as secondary fermenters related to sulfate or iron reducers might be responsible for hydrogen production in the fen. Comparative analysis of [Fe-Fe]-hydrogenase and 16S rRNA gene-based phylogenies indicated the presence of homologous multiple hydrogenases per organism and inconsistencies between 16S rRNA gene- and [Fe-Fe]-hydrogenase-based phylogenies, necessitating appropriate qualification of [Fe-Fe]-hydrogenase gene data for diversity analyses.

External Organisation(s)

University of Bayreuth

Type

Article

Journal

Applied and Environmental Microbiology

Volume

76

Pages

2027-2031

No. of pages

5

ISSN

0099-2240

Publication date

03.2010

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Biotechnology, Food Science, Applied Microbiology and Biotechnology, Ecology

Sustainable Development Goals

SDG 7 - Affordable and Clean Energy

Electronic version(s)

https://doi.org/10.1128/AEM.02895-09 (Access: Open)
https://doi.org/10.15488/15921 (Access: Open)