Protein microarrays: Reducedautofluorescence and improved LOD

authored by: Johanna Gabriela Walter, Frank Stahl, Michael Reck, Inka Praulich, Yakir Nataf, Markus Hollas, Karl Pflanz, Dieter Melzner, Yuval Shoham, Thomas Scheper
Abstract: In protein microarray performance, the choice of an appropriate surface is a crucial factor. Three-dimensional substrates like nitrocellulose are known to have higher binding capacities than planar surfaces. Furthermore, they can enable the immobilization of proteins in a functional manner. One disadvantage of today's nitrocellulose-based microarrays is the high background fluorescence, which can interfere with the detection of low-abundance proteins. We have developed an innovative black nitrocellulose membrane-based protein microarray that exhibits low autofluorescence in combination with increased sensitivity and improved LOD (limit of detection). The applicability of the novel material was demonstrated with main focus on reversed-phase microarray experiments. In comparison to various commercially available microarrays, a higher sensitivity in regard to the spotted protein was achieved. In contrast to other porous nitrocellulose-based microarrays, the black nitrocellulose provides a significant lower autofluorescence and background intensity.
Organisation(s): Institute of Technical Chemistry
External Organisation(s): Technion-Israel Institute of Technology
Sartorius AG
Type: Article
Journal: Engineering in life sciences
Volume: 10
Pages: 103-108
No. of pages: 6
ISSN: 1618-0240
Publication date: 12.04.2010
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Biotechnology, Environmental Engineering, Bioengineering
Electronic version(s): https://doi.org/10.1002/elsc.200900078 (Access: Unknown)

BibTeX

@article{e5cf0418927a4f4ca95a8f01b2052fe6,
title = "Protein microarrays: Reducedautofluorescence and improved LOD",
abstract = "In protein microarray performance, the choice of an appropriate surface is a crucial factor. Three-dimensional substrates like nitrocellulose are known to have higher binding capacities than planar surfaces. Furthermore, they can enable the immobilization of proteins in a functional manner. One disadvantage of today's nitrocellulose-based microarrays is the high background fluorescence, which can interfere with the detection of low-abundance proteins. We have developed an innovative black nitrocellulose membrane-based protein microarray that exhibits low autofluorescence in combination with increased sensitivity and improved LOD (limit of detection). The applicability of the novel material was demonstrated with main focus on reversed-phase microarray experiments. In comparison to various commercially available microarrays, a higher sensitivity in regard to the spotted protein was achieved. In contrast to other porous nitrocellulose-based microarrays, the black nitrocellulose provides a significant lower autofluorescence and background intensity.",
author = "Walter, {Johanna Gabriela} and Frank Stahl and Michael Reck and Inka Praulich and Yakir Nataf and Markus Hollas and Karl Pflanz and Dieter Melzner and Yuval Shoham and Thomas Scheper",
year = "2010",
month = apr,
day = "12",
doi = "10.1002/elsc.200900078",
language = "English",
volume = "10",
pages = "103--108",
journal = "Engineering in life sciences",
issn = "1618-0240",
publisher = "Wiley-VCH Verlag",
number = "2",
}