Sour cherry strain of plum pox potyvirus (PPV)

Molecular and serological evidence for a new subgroup of PPV strains

authored by: L. Nemchinov, A. Hadidi, E. Maiss, M. Cambra, T. Candresse, V. Damsteegt
Abstract: Properties of the unusual sour cherry strain of plum pox potyvirus (PPV- SoC) were investigated by sequencing its 3'-terminal 1,360 nt and examining its serological reactivity with several monoclonal antibodies (MAbs) to PPV. A significantly low degree of identity of the sequenced region has been found between PPV-SoC and other isolates of PPV. This is due to the high nucleotide divergence between the 5'-terminal region of the coat protein (CP) coding region of PPV-SoC and that of other isolates. Most nucleotide substitutions in the sequenced region are not silent and result in amino acid changes, especially near the N terminus of the CP. The unique 5' terminus of the PPV- SoC CP coding sequence has been utilized to develop a cRNA probe that hybridizes to PPV-SoC but not to other isolates/members of the D or M subgroups of PPV. The N-terminal region of the PPV-SoC CP contains the recognizable DAG motif that determines aphid transmissibility of potyviruses. Aphid transmission of PPV-SoC to herbaceous and woody hosts has been confirmed experimentally. Serological reactivity of PPV-SoC with 10 MAbs of PPV suggest that PPV-SoC represents a new serotype of PPV that does not fall into the conventional D or M serotypes/subgroups. We propose that PPV-SoC represents a new subgroup of PPV strains, termed PPV cherry (PPV-C), and that PPV-SoC is its prototype member.
Organisation(s): Phytomedicine Section
External Organisation(s): U.S. Department of Agriculture
Instituto Valenciano de Investigaciones Agrarias
Institut national de recherche pour l’agriculture, l’alimentation et l’environnement (INRAE)
Type: Article
Journal: PHYTOPATHOLOGY
Volume: 86
Pages: 1215-1221
No. of pages: 7
ISSN: 0031-949X
Publication date: 11.1996
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Agronomy and Crop Science, Plant Science
Electronic version(s): https://doi.org/10.1094/Phyto-86-1215 (Access: Closed)

BibTeX

@article{c4b104918b084a998b4ca0bd030fb02c,
title = "Sour cherry strain of plum pox potyvirus (PPV): Molecular and serological evidence for a new subgroup of PPV strains",
abstract = "Properties of the unusual sour cherry strain of plum pox potyvirus (PPV- SoC) were investigated by sequencing its 3'-terminal 1,360 nt and examining its serological reactivity with several monoclonal antibodies (MAbs) to PPV. A significantly low degree of identity of the sequenced region has been found between PPV-SoC and other isolates of PPV. This is due to the high nucleotide divergence between the 5'-terminal region of the coat protein (CP) coding region of PPV-SoC and that of other isolates. Most nucleotide substitutions in the sequenced region are not silent and result in amino acid changes, especially near the N terminus of the CP. The unique 5' terminus of the PPV- SoC CP coding sequence has been utilized to develop a cRNA probe that hybridizes to PPV-SoC but not to other isolates/members of the D or M subgroups of PPV. The N-terminal region of the PPV-SoC CP contains the recognizable DAG motif that determines aphid transmissibility of potyviruses. Aphid transmission of PPV-SoC to herbaceous and woody hosts has been confirmed experimentally. Serological reactivity of PPV-SoC with 10 MAbs of PPV suggest that PPV-SoC represents a new serotype of PPV that does not fall into the conventional D or M serotypes/subgroups. We propose that PPV-SoC represents a new subgroup of PPV strains, termed PPV cherry (PPV-C), and that PPV-SoC is its prototype member.",
keywords = "detection, differentiation, insect transmission, nucleotide sequence of coat protein, phylogeny",
author = "L. Nemchinov and A. Hadidi and E. Maiss and M. Cambra and T. Candresse and V. Damsteegt",
year = "1996",
month = nov,
doi = "10.1094/Phyto-86-1215",
language = "English",
volume = "86",
pages = "1215--1221",
journal = "PHYTOPATHOLOGY",
issn = "0031-949X",
publisher = "American Phytopathological Society",
number = "11",
}