Adaptation of in-situ microscopy for crystallization processes

authored by: A. Bluma, T. Höpfner, G. Rudolph, P. Lindner, S. Beutel, B. Hitzmann, T. Scheper
Abstract: In biotechnological and pharmaceutical engineering, the study of crystallization processes gains importance. An efficient analytical inline sensor could help to improve the knowledge about these processes in order to increase efficiency and yields. The in-situ microscope (ISM) is an optical sensor developed for the monitoring of bioprocesses. A new application for this sensor is the monitoring in downstream processes, e.g. the crystallization of proteins and other organic compounds. This contribution shows new aspects of using in-situ microscopy to monitor crystallization processes. Crystals of different chemical compounds were precipitated from supersaturated solutions and the crystal growth was monitored. Exemplified morphological properties and different forms of crystals could be distinguished on the basis of offline experiments. For inline monitoring of crystallization processes, a special 0.5 L stirred tank reactor was developed and equipped with the in-situ microscope. This reactor was utilized to carry out batch experiments for crystallizations of O-acetylsalicyclic acid (ASS) and hen egg white lysozyme (HEWL). During the whole crystallization process, the in-situ microscope system acquired images directly from the crystallization broth. For the data evaluation, an image analysis algorithm was developed and implemented in the microscope analysis software.
Organisation(s): Institute of Technical Chemistry
Type: Article
Journal: Journal of crystal growth
Volume: 311
Pages: 4193-4198
No. of pages: 6
ISSN: 0022-0248
Publication date: 09.07.2009
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Condensed Matter Physics, Inorganic Chemistry, Materials Chemistry
Electronic version(s): https://doi.org/10.1016/j.jcrysgro.2009.06.057 (Access: Unknown)

BibTeX

@article{c3f8aed3c30d41d49be5b1415ec94f4c,
title = "Adaptation of in-situ microscopy for crystallization processes",
abstract = "In biotechnological and pharmaceutical engineering, the study of crystallization processes gains importance. An efficient analytical inline sensor could help to improve the knowledge about these processes in order to increase efficiency and yields. The in-situ microscope (ISM) is an optical sensor developed for the monitoring of bioprocesses. A new application for this sensor is the monitoring in downstream processes, e.g. the crystallization of proteins and other organic compounds. This contribution shows new aspects of using in-situ microscopy to monitor crystallization processes. Crystals of different chemical compounds were precipitated from supersaturated solutions and the crystal growth was monitored. Exemplified morphological properties and different forms of crystals could be distinguished on the basis of offline experiments. For inline monitoring of crystallization processes, a special 0.5 L stirred tank reactor was developed and equipped with the in-situ microscope. This reactor was utilized to carry out batch experiments for crystallizations of O-acetylsalicyclic acid (ASS) and hen egg white lysozyme (HEWL). During the whole crystallization process, the in-situ microscope system acquired images directly from the crystallization broth. For the data evaluation, an image analysis algorithm was developed and implemented in the microscope analysis software.",
keywords = "A1. Biocrystallization, A2. Crystal growth, A2. Crystal morphology, B1. ASS, B1. Lysozyme, B3. In-situ microscopy",
author = "A. Bluma and T. H{\"o}pfner and G. Rudolph and P. Lindner and S. Beutel and B. Hitzmann and T. Scheper",
note = "Funding information: The authors would like to thank the DFG for supporting the Project SCHE279/23-1.",
year = "2009",
month = jul,
day = "9",
doi = "10.1016/j.jcrysgro.2009.06.057",
language = "English",
volume = "311",
pages = "4193--4198",
journal = "Journal of crystal growth",
issn = "0022-0248",
publisher = "Elsevier",
number = "17",
}