A Rotating Bed System Bioreactor Enables Cultivation of Primary Osteoblasts onWell-Characterized Sponceram® Regarding Structural and Flow Properties

authored by: Kirstin Suck, Stefanie Roeker, Solvig Diederichs, Fabienne Anton, Jose A. Sanz-Herrera, Ignacio Ochoa, Manuel Doblare, Thomas Scheper, Martijn van Griensven, Cornelia Kasper
Abstract: The development of bone tissue engineering depends on the availability of suitable biomaterials, a well-defined and controlled bioreactor system, and on the use of adequate cells. The biomaterial must fulfill chemical, biological, and mechanical requirements. Besides biocompatibility, the structural and flow characteristics of the biomaterial are of utmost importance for a successful dynamic cultivation of osteoblasts, since fluid percolation within the microstructure must be assured to supply to cells nutrients and waste removal. Therefore, the biomaterial must consist of a three-dimensional structure, exhibit high porosity and present an interconnected porous network. Sponceram®, a ZrO₂ based porous ceramic, is characterized in the presented work with regard to its microstructural design. Intrinsic permeability is obtained through a standard Darcy's experiment, while Young's modulus is derived from a two plates stress-strain test in the linear range. Furthermore, the material is applied for the dynamic cultivation of primary osteoblasts in a newly developed rotating bed bioreactor.
Organisation(s): Institute of Technical Chemistry
External Organisation(s): Universidad de Zaragoza
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology
Type: Article
Journal: Biotechnology progress
Volume: 26
Pages: 671-678
No. of pages: 8
ISSN: 8756-7938
Publication date: 29.01.2010
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Biotechnology
Electronic version(s): https://doi.org/10.1002/btpr.386 (Access: Unknown)

BibTeX

@article{c16c17a743ef441f93c664c8a7ba892d,
title = "A Rotating Bed System Bioreactor Enables Cultivation of Primary Osteoblasts onWell-Characterized Sponceram{\textregistered} Regarding Structural and Flow Properties",
abstract = "The development of bone tissue engineering depends on the availability of suitable biomaterials, a well-defined and controlled bioreactor system, and on the use of adequate cells. The biomaterial must fulfill chemical, biological, and mechanical requirements. Besides biocompatibility, the structural and flow characteristics of the biomaterial are of utmost importance for a successful dynamic cultivation of osteoblasts, since fluid percolation within the microstructure must be assured to supply to cells nutrients and waste removal. Therefore, the biomaterial must consist of a three-dimensional structure, exhibit high porosity and present an interconnected porous network. Sponceram{\textregistered}, a ZrO2 based porous ceramic, is characterized in the presented work with regard to its microstructural design. Intrinsic permeability is obtained through a standard Darcy's experiment, while Young's modulus is derived from a two plates stress-strain test in the linear range. Furthermore, the material is applied for the dynamic cultivation of primary osteoblasts in a newly developed rotating bed bioreactor.",
keywords = "Biomechanics, Primary osteoblasts, Rotating bed system, Sponceram{\textregistered}, Young's modulus",
author = "Kirstin Suck and Stefanie Roeker and Solvig Diederichs and Fabienne Anton and Sanz-Herrera, {Jose A.} and Ignacio Ochoa and Manuel Doblare and Thomas Scheper and {van Griensven}, Martijn and Cornelia Kasper",
year = "2010",
month = jan,
day = "29",
doi = "10.1002/btpr.386",
language = "English",
volume = "26",
pages = "671--678",
journal = "Biotechnology progress",
issn = "8756-7938",
publisher = "Wiley-Blackwell",
number = "3",
}