ENZYMATIC PRODUCTION OF L-AMINO ACID WITH CONTINUOUS COENZYME REGENERATION BY LIQUID MEMBRANE TECHNIQUE.

authored by

Kyriakos Makryaleas, Thomas Scheper, Karl Schuegerl, Maria Regina Kula

Abstract

L-Leucine-dehydrogenase (LEUDH) catalyzes the reductive amination of alpha -ketoisocaproate to L-leucine. During this reaction, NADH is oxidized and simultaneously reduced by formatedehydrogenase (FDH). The two enzymes and the coenzyme were immobilized in a liquid membrane emulsion. Several organic phases and emulsifiers were tested, in order to obtain suitable immobilization systems. Batch tests were carried out for optimization of operating conditions and determination of kinetic parameters. Mass transfer through the membrane is the rate determining step. Permeation coefficients for the substrates and product were determined on solid-supported liquid membranes.

Organisation(s)

Institute of Technical Chemistry

Type

Article

Journal

German Chemical Engineering

Volume

8

Pages

345-350

No. of pages

6

ISSN

0343-5539

Publication date

11.1985

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

General Engineering