The modular nodulins Nvf 28/32 of broad bean (Vicia faba L.)

Alternative exon combinations account far different modular structures

authored by: Helge Küster, Martin Frühling, Alfred Pühler, Andreas M. Perlick
Abstract: The broad bean late nodulins, Nvf-28/32, are composed of two types of repetitively occurring sequence modules flanked by unique N- and C-terminal modules. Six isoforms of these nodulins were characterized by a specific modular structure resulting from a different individual order of repetitive sequence modules. A detailed analysis of genomic PCR fragments revealed that the repetitive modules and the N-terminal unique module exactly corresponded to exons, whereas the C-terminal module was specified by two exons. Since those exons encoding the repetitive modules missing in specific Nvf-28/32 isoforms were consistently present within genomic sequences, a post-transcriptional generation of VfNOD28/32 transcripts specifying six Nvf-28/32 nodulins was concluded. Using tissue-print hybridizations, these transcripts were localized in the interzone II-III and the nitrogen-fixing zone III of root nodules. From this and from cDNA-cDNA hybridizations demonstrating a comparable timing of expression of VfNOD28/32 and of leghemoglobin transcripts in root nodules, a function of the modular nodulins Nvf-28/32 in late developmental stages of broad bean nodules was inferred.
External Organisation(s): Bielefeld University
Type: Article
Journal: Molecular and General Genetics
Volume: 252
Pages: 648-657
No. of pages: 10
ISSN: 0026-8925
Publication date: 21.11.1996
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Genetics
Electronic version(s): https://doi.org/10.1007/s004380050273 (Access: Unknown)

BibTeX

@article{aef515dd056647fb915e8003671e66d7,
title = "The modular nodulins Nvf 28/32 of broad bean (Vicia faba L.): Alternative exon combinations account far different modular structures",
abstract = "The broad bean late nodulins, Nvf-28/32, are composed of two types of repetitively occurring sequence modules flanked by unique N- and C-terminal modules. Six isoforms of these nodulins were characterized by a specific modular structure resulting from a different individual order of repetitive sequence modules. A detailed analysis of genomic PCR fragments revealed that the repetitive modules and the N-terminal unique module exactly corresponded to exons, whereas the C-terminal module was specified by two exons. Since those exons encoding the repetitive modules missing in specific Nvf-28/32 isoforms were consistently present within genomic sequences, a post-transcriptional generation of VfNOD28/32 transcripts specifying six Nvf-28/32 nodulins was concluded. Using tissue-print hybridizations, these transcripts were localized in the interzone II-III and the nitrogen-fixing zone III of root nodules. From this and from cDNA-cDNA hybridizations demonstrating a comparable timing of expression of VfNOD28/32 and of leghemoglobin transcripts in root nodules, a function of the modular nodulins Nvf-28/32 in late developmental stages of broad bean nodules was inferred.",
keywords = "Alternative splicing, Late nodulins, Tissue-print hybridization",
author = "Helge K{\"u}ster and Martin Fr{\"u}hling and Alfred P{\"u}hler and Perlick, {Andreas M.}",
year = "1996",
month = nov,
day = "21",
doi = "10.1007/s004380050273",
language = "English",
volume = "252",
pages = "648--657",
journal = "Molecular and General Genetics",
issn = "0026-8925",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "6",
}