Bio-field-effect transistors as detectors in flow-injection analysis

authored by: U. Brand, B. Reinhardt, F. Rüther, T. Scheper, K. Schügerl
Abstract: The development of enzyme-modified bio-field-effect transistors (BioFETs) for the determination of glucose, urea, penicillin G, penicillin V and cephalosporin C is reported. BioFETs are produced by covering the pH-sensitive gate areas of ion-selective field-effect transistors with enzyme membranes. The characteristics of the resulting BioFETs and the influence of several parameters, e.g., pH and buffer capacity, are described. The measuring range covers 1-2 orders of magnitude of substrate concentration, and the BioFETs are applicable for 3-12 weeks, depending on the enzyme. They show a short response time and are well suited for detection in flow systems. The frequency of determination with BioFETs in flow systems is high (15-20 measurements per hour). The application of a BioFET in on-line bioprocess control is described. A glucose oxidase FET monitors the glucose concentration during cultivation of Escherichia coli. The results correspond well with off-line liquid chromatographic determinations.
Organisation(s): Institute of Technical Chemistry
Type: Article
Journal: Analytica chimica acta
Volume: 238
Pages: 201-210
No. of pages: 10
ISSN: 0003-2670
Publication date: 1990
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Analytical Chemistry, Biochemistry, Environmental Chemistry, Spectroscopy
Electronic version(s): https://doi.org/10.1016/S0003-2670(00)80538-3 (Access: Unknown)

BibTeX

@article{a00c94f50789481f93b0e129f0b76eba,
title = "Bio-field-effect transistors as detectors in flow-injection analysis",
abstract = "The development of enzyme-modified bio-field-effect transistors (BioFETs) for the determination of glucose, urea, penicillin G, penicillin V and cephalosporin C is reported. BioFETs are produced by covering the pH-sensitive gate areas of ion-selective field-effect transistors with enzyme membranes. The characteristics of the resulting BioFETs and the influence of several parameters, e.g., pH and buffer capacity, are described. The measuring range covers 1-2 orders of magnitude of substrate concentration, and the BioFETs are applicable for 3-12 weeks, depending on the enzyme. They show a short response time and are well suited for detection in flow systems. The frequency of determination with BioFETs in flow systems is high (15-20 measurements per hour). The application of a BioFET in on-line bioprocess control is described. A glucose oxidase FET monitors the glucose concentration during cultivation of Escherichia coli. The results correspond well with off-line liquid chromatographic determinations.",
keywords = "Biosensors, Cephalosporin C, Field-effect transistors, Glucose, Penicillin, Process analysis, Urea",
author = "U. Brand and B. Reinhardt and F. R{\"u}ther and T. Scheper and K. Sch{\"u}gerl",
note = "Funding information: The authors thank Dr. R. Howind of Eppen-dorf, Hamburg, for his cooperation concerning the FIA system. The financial support of the Bundesministerium fur Forschung und Technolo-gie of the Federal Republic of Germany is gratefully acknowledged.",
year = "1990",
doi = "10.1016/S0003-2670(00)80538-3",
language = "English",
volume = "238",
pages = "201--210",
journal = "Analytica chimica acta",
issn = "0003-2670",
publisher = "Elsevier",
number = "C",
}