Efficient and stable regeneration from protoplasts of Cyclamen coum Miller via somatic embryogenesis

authored by: Anika Nadja Sabine Prange, Margrethe Serek, Melanie Bartsch, Traud Winkelmann
Abstract: Embryogenic cultures of Cyclamen coum were established on solid media and in suspension, and their growth characteristics in response to different concentrations of plant growth regulators (PGRs) were evaluated. Embryogenic cultures exhibited a high regeneration capacity of 876 somatic embryos per gram fresh mass. Up to 4.24 × 10⁵ protoplasts per gram of fresh mass were isolated from somatic embryos and embryogenic suspension cultures. Protoplasts derived from both embryos and suspension cultures were successfully cultured in vitro and regenerated into plants via somatic embryogenesis. Phenotypic analyses and flow cytometric measurements revealed that some regenerated plants were tetraploid. About 20% of the protoplast-derived calluses used for regeneration were tetraploid, while tetraploidy was found in 0.9% of the plants regenerated from the embryogenic cultures.
Organisation(s): Section Reproduction and Development
Type: Article
Journal: Plant Cell, Tissue and Organ Culture
Volume: 101
Pages: 171-182
No. of pages: 12
ISSN: 0167-6857
Publication date: 04.02.2010
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Horticulture
Electronic version(s): https://doi.org/10.1007/s11240-010-9674-z (Access: Closed)

BibTeX

@article{98de7b77f6c943eea311990f143761a2,
title = "Efficient and stable regeneration from protoplasts of Cyclamen coum Miller via somatic embryogenesis",
abstract = "Embryogenic cultures of Cyclamen coum were established on solid media and in suspension, and their growth characteristics in response to different concentrations of plant growth regulators (PGRs) were evaluated. Embryogenic cultures exhibited a high regeneration capacity of 876 somatic embryos per gram fresh mass. Up to 4.24 × 105 protoplasts per gram of fresh mass were isolated from somatic embryos and embryogenic suspension cultures. Protoplasts derived from both embryos and suspension cultures were successfully cultured in vitro and regenerated into plants via somatic embryogenesis. Phenotypic analyses and flow cytometric measurements revealed that some regenerated plants were tetraploid. About 20% of the protoplast-derived calluses used for regeneration were tetraploid, while tetraploidy was found in 0.9% of the plants regenerated from the embryogenic cultures.",
keywords = "Flow cytometry, Ornamental plant, Ploidy stability, Protoplast isolation, Somaclonal variation, Vegetative propagation",
author = "Prange, {Anika Nadja Sabine} and Margrethe Serek and Melanie Bartsch and Traud Winkelmann",
note = "Funding Information: Acknowledgments The authors thank Frank Schaarschmidt for his excellent guidance in the statistical data analysis. The financial support of the German Federal Ministry of Economics and Technology through the program PRO INNO grant no. KF0054802MD5 is gratefully acknowledged.",
year = "2010",
month = feb,
day = "4",
doi = "10.1007/s11240-010-9674-z",
language = "English",
volume = "101",
pages = "171--182",
journal = "Plant Cell, Tissue and Organ Culture",
issn = "0167-6857",
publisher = "Springer Netherlands",
number = "2",
}