Dynamic Cultivation of Human Mesenchymal Stem Cells in a Rotating BedBioreactor System Based on the Z®RP Platform

authored by: Solvig Diederichs, Stefanie Röker, Dana Marten, Anja Peterbauer, Thomas Scheper, Martijn van Griensven, Cornelia Kasper
Abstract: Because the regeneration of large bone defects is limited by quantitative restrictions and risks of infections, the development of bioartificial bone substitutes is of great importance. To obtain a three-dimensional functional tissue-like graft, static cultivation is inexpedient due to limitations in cell density, nutrition and oxygen support. Dynamic cultivation in a bioreactor system can overcome these restrictions and furthermore provide the possibility to control the environment with regard to pH, oxygen content, and temperature. In this study, a three-dimensional bone construct was engineered by the use of dynamic bioreactor technology. Human adipose tissue derived mesenchymal stem cells were cultivated on a macroporous zirconium dioxide based ceramic disc called Sponceram®. Furthermore, hydroxyapatite coated Sponceram® was used. The cells were cultivated under dynamic conditions and compared with statically cultivated cells. The differentiation into osteoblasts was initiated by osteogenic supplements. Cellular proliferation during static and dynamic cultivation was compared measuring glucose and lactate concentration. The differentiation process was analysed determining AP-expression and using different specific staining methods. Our results demonstrate much higher proliferation rates during dynamic conditions in the bioreactor system compared to static cultivation measured by glucose consumption and lactate production. Cell densities on the scaffolds indicated higher proliferation on native Sponceram® compared to hydroxyapatite coated Sponceram®. With this study, we present an excellent method to enhance cellular proliferation and bone lineage specific growth of tissue like structures comprising fibrous (collagen) and globular (mineral) extracellular components.
Organisation(s): Institute of Technical Chemistry
External Organisation(s): Red Cross Blood Service of Upper Austria
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology
Type: Article
Journal: Biotechnology progress
Volume: 25
Pages: 1762-1771
No. of pages: 10
ISSN: 8756-7938
Publication date: 11.2009
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Biotechnology
Electronic version(s): https://doi.org/10.1002/btpr.258 (Access: Unknown)

BibTeX

@article{38257bb4b707486a93a01c0a8f886d32,
title = "Dynamic Cultivation of Human Mesenchymal Stem Cells in a Rotating BedBioreactor System Based on the Z{\textregistered}RP Platform",
abstract = "Because the regeneration of large bone defects is limited by quantitative restrictions and risks of infections, the development of bioartificial bone substitutes is of great importance. To obtain a three-dimensional functional tissue-like graft, static cultivation is inexpedient due to limitations in cell density, nutrition and oxygen support. Dynamic cultivation in a bioreactor system can overcome these restrictions and furthermore provide the possibility to control the environment with regard to pH, oxygen content, and temperature. In this study, a three-dimensional bone construct was engineered by the use of dynamic bioreactor technology. Human adipose tissue derived mesenchymal stem cells were cultivated on a macroporous zirconium dioxide based ceramic disc called Sponceram{\textregistered}. Furthermore, hydroxyapatite coated Sponceram{\textregistered} was used. The cells were cultivated under dynamic conditions and compared with statically cultivated cells. The differentiation into osteoblasts was initiated by osteogenic supplements. Cellular proliferation during static and dynamic cultivation was compared measuring glucose and lactate concentration. The differentiation process was analysed determining AP-expression and using different specific staining methods. Our results demonstrate much higher proliferation rates during dynamic conditions in the bioreactor system compared to static cultivation measured by glucose consumption and lactate production. Cell densities on the scaffolds indicated higher proliferation on native Sponceram{\textregistered} compared to hydroxyapatite coated Sponceram{\textregistered}. With this study, we present an excellent method to enhance cellular proliferation and bone lineage specific growth of tissue like structures comprising fibrous (collagen) and globular (mineral) extracellular components.",
keywords = "Bioreactor, Bone tissue engineering, Dynamic mesenchymal stem cell cultivation, Rotating bed system, Sponceram{\textregistered}",
author = "Solvig Diederichs and Stefanie R{\"o}ker and Dana Marten and Anja Peterbauer and Thomas Scheper and {van Griensven}, Martijn and Cornelia Kasper",
year = "2009",
month = nov,
doi = "10.1002/btpr.258",
language = "English",
volume = "25",
pages = "1762--1771",
journal = "Biotechnology progress",
issn = "8756-7938",
publisher = "Wiley-Blackwell",
number = "6",
}