Polymorphism and gene arrangement among plastomes of ten Epilobium species

authored by: Udo Schmitz, Klaus V. Kowallik
Abstract: Plastid DNAs of ten different Epilobium species from four continents have been analysed using the restriction endonucleases BamHI, BglI, BglII, EcoRI, PstI, PvuII and SalI. With respect to the position of cleavage sites of those enzymes, each species has a specific plastome. Fragment patterns of different species from the same continent show a higher degree of similarity than those from different continents. Physical maps of the circular plastid DNA molecule have been constructed for each of the ten species by localising the cleavage sites of the enzymes BglI, PvuII and SalI. As in most other higher plants, the plastid DNA of Epilobium is segmentally organized into two inverted repeats separated by a large and a small single copy region. In heterologous hybridization experiments using radioactively labelled gene probes, the positions of structural genes coding for the rRNAs and for seven polypeptides have been determined. In contrast to its closest relative, Oenothera, the gene arrangement of Epilobium plastomes has the same order as in spinach. This indicates that changes in gene arrangement may be genus-specific and not the result of one or several events affecting all members of a plant family.
External Organisation(s): University Hospital Düsseldorf
Type: Article
Journal: Plant Molecular Biology
Volume: 7
Pages: 115-127
No. of pages: 13
ISSN: 0167-4412
Publication date: 03.1986
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Agronomy and Crop Science, Genetics, Plant Science
Electronic version(s): https://doi.org/10.1007/BF00040138 (Access: Unknown)

BibTeX

@article{0735c27345d34b3d87bccf8681631df4,
title = "Polymorphism and gene arrangement among plastomes of ten Epilobium species",
abstract = "Plastid DNAs of ten different Epilobium species from four continents have been analysed using the restriction endonucleases BamHI, BglI, BglII, EcoRI, PstI, PvuII and SalI. With respect to the position of cleavage sites of those enzymes, each species has a specific plastome. Fragment patterns of different species from the same continent show a higher degree of similarity than those from different continents. Physical maps of the circular plastid DNA molecule have been constructed for each of the ten species by localising the cleavage sites of the enzymes BglI, PvuII and SalI. As in most other higher plants, the plastid DNA of Epilobium is segmentally organized into two inverted repeats separated by a large and a small single copy region. In heterologous hybridization experiments using radioactively labelled gene probes, the positions of structural genes coding for the rRNAs and for seven polypeptides have been determined. In contrast to its closest relative, Oenothera, the gene arrangement of Epilobium plastomes has the same order as in spinach. This indicates that changes in gene arrangement may be genus-specific and not the result of one or several events affecting all members of a plant family.",
keywords = "DNA polymorphism, Epilobium, gene rearrangement, plastid DNA, plastome evolution, restriction mapping",
author = "Udo Schmitz and Kowallik, {Klaus V.}",
year = "1986",
month = mar,
doi = "10.1007/BF00040138",
language = "English",
volume = "7",
pages = "115--127",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer Netherlands",
number = "2",
}