The effect of an rhBMP-2 absorbable collagen sponge-targeted system on bone formation in vivo

verfasst von: Rick Visser, Pilar M. Arrabal, Jose Becerra, Ursula Rinas, Manuel Cifuentes
Abstract: Reparation of bone defects remains a major clinical and economic concern, with more than 3 million bone grafts performed annually only in the United States and the EU. The search for alternatives to autologous bone grafting led to the approval by the FDA of an absorbable collagen carrier combined with rhBMP-2 for the treatment of certain bone diseases and fractures. The present work is focused on the production of a collagen-targeted rhBMP-2 based system to improve bone formation. We produced a modified rhBMP-2 with only an additional collagen-binding decapeptide derived from the von Willebrand factor and tested its affinity to collagen and its ability to induce ectopic bone formation in vivo when implanted in combination with absorbable collagen sponges or hydroxyapatite. The results showed not only that the rhBMP2-CBD had an increased affinity to collagen, but also that this binding was very stable during a prolonged period of time. In vivo experiments demonstrated that this rhBMP2-CBD maintained its osteoinductive activity, being capable of inducing new bone formation even at lower concentrations than native rhBMP-2. These results indicate that the combination of the fusion protein with absorbable collagen may be a suitable and safer alternative to rhBMP-2 for bone repair purposes.
Externe Organisation(en): Universidad de Malaga
Centros de Investigacion Biomedica en Red - CIBER
Helmholtz-Zentrum für Infektionsforschung GmbH (HZI)
Typ: Artikel
Journal: BIOMATERIALS
Band: 30
Seiten: 2032-2037
Anzahl der Seiten: 6
ISSN: 0142-9612
Publikationsdatum: 04.2009
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
ASJC Scopus Sachgebiete: Bioengineering, Keramische und Verbundwerkstoffe, Biophysik, Biomaterialien, Werkstoffmechanik
Elektronische Version(en): https://doi.org/10.1016/j.biomaterials.2008.12.046 (Zugang: Geschlossen)

BibTeX

@article{f4154dce4a7044e3b72bb06c46584e7a,
title = "The effect of an rhBMP-2 absorbable collagen sponge-targeted system on bone formation in vivo",
abstract = "Reparation of bone defects remains a major clinical and economic concern, with more than 3 million bone grafts performed annually only in the United States and the EU. The search for alternatives to autologous bone grafting led to the approval by the FDA of an absorbable collagen carrier combined with rhBMP-2 for the treatment of certain bone diseases and fractures. The present work is focused on the production of a collagen-targeted rhBMP-2 based system to improve bone formation. We produced a modified rhBMP-2 with only an additional collagen-binding decapeptide derived from the von Willebrand factor and tested its affinity to collagen and its ability to induce ectopic bone formation in vivo when implanted in combination with absorbable collagen sponges or hydroxyapatite. The results showed not only that the rhBMP2-CBD had an increased affinity to collagen, but also that this binding was very stable during a prolonged period of time. In vivo experiments demonstrated that this rhBMP2-CBD maintained its osteoinductive activity, being capable of inducing new bone formation even at lower concentrations than native rhBMP-2. These results indicate that the combination of the fusion protein with absorbable collagen may be a suitable and safer alternative to rhBMP-2 for bone repair purposes.",
keywords = "BMP (bone morphogenetic protein), Bone tissue engineering, Collagen, Osteogenesis, Recombinant protein",
author = "Rick Visser and Arrabal, {Pilar M.} and Jose Becerra and Ursula Rinas and Manuel Cifuentes",
note = "Funding Information: The authors would like to thank Ms. Silvia Hern{\'a}ndez and Ms. Eva Jim{\'e}nez for technical support. Thanks also to Dr. Luis Felipe Vallejo for his valuable help in protein production and refolding. This work was partially supported by Red TerCel RD06/0010/0014 (Ministerio de Sanidad y Consumo), BIO2006-03599 (Ministerio de Educaci{\'o}n y Ciencia), TCRM 0012/2006 (Consejer{\'i}a de Salud, Junta de Andaluc{\'i}a) and P07-CVI-02781 (Consejer{\'i}a de Innovaci{\'o}n, Ciencia y Empresa, Junta de Andaluc{\'i}a). ",
year = "2009",
month = apr,
doi = "10.1016/j.biomaterials.2008.12.046",
language = "English",
volume = "30",
pages = "2032--2037",
journal = "BIOMATERIALS",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "11",
}