Transesterification of phosphodiester by a zinc-containing cyclen derivative
Identification of the active species
- verfasst von
- Markus Kalesse, Andrea Loos
- Abstract
The active site analogue 2 of alkaline phosphatase was synthesized and its hydrolytic activity was tested. Efficient hydrolysis was observed at pH > 8 at various concentrations of the hydrolytic species. The cyclic phosphate 3 derived from transesterification could be identified by 31P-NMR spectroscopy. A comparison of the hydrolysis results with the species distribution curve revealed the ZnL-OH species as the active complex. The complex stability constant (log KZnL) of ligand 7 was determined to be 13.7 (± 0.5). K′ for the hydrolysis of the p-nitrophenyl phosphate ester of propylene glycol (1) with 2 (0.02 M, pH = 8.35) is 1.5 × 10-3 s-1 at 50°C.
- Organisationseinheit(en)
-
Institut für Organische Chemie
- Typ
- Artikel
- Journal
- Liebigs Annales
- Seiten
- 935-939
- Anzahl der Seiten
- 5
- ISSN
- 0947-3440
- Publikationsdatum
- 06.1996
- Publikationsstatus
- Veröffentlicht
- Peer-reviewed
- Ja
- ASJC Scopus Sachgebiete
- Allgemeine Chemie
- Elektronische Version(en)
-
https://doi.org/10.1002/jlac.199619960611 (Zugang:
Unbekannt)
