Unspecific peroxygenase immobilization in 3D-printed microfluidics

towards tailor-made screening platforms

verfasst von

Elena Gkantzou, Theofilia Koulopoulou, Hannah Brass, David Schönauer, Anton Glieder, Selin Kara

Abstract

In the context of empowering biocatalysis, an easy-to-develop, reproducible, and easy-to-scale biocatalytic system under continuous flow is demonstrated. 3D printing technology is used as the reactor manufacturing method, yielding identical and low-cost microfluidic chips that can be further modified to serve as a biocatalytic platform for simultaneous parameter screening. The model enzyme studied here is unspecific peroxygenase (UPO). UPOs are currently under intensive study, due to their distinct promiscuity in oxyfunctionalization chemistry. This is the first study demonstrating UPO's immobilization in a microfluidic concept. The developed method for surface functionalization of microfluidic reactors is based on polydopamine modification and was proven highly reproducible. UPO showed a TTN of 19 249 and a STY of 2.1 g L

^-1 h

^-1, under the specified conditions. The kinetic behavior of the system under flow conditions is reported. The system was also regenerated with a 51.4% recovered activity. Further utilization of microfluidic concepts is expected to unravel the full potential of UPOs for oxyfunctionalization reactions of particular interest. The proposed system is foreseen as a screening platform for different reaction conditions, reaction substrates, or enzyme mutants.

Organisationseinheit(en)

Institut für Technische Chemie

Externe Organisation(en)

Bisy GmbH
Aarhus University

Typ

Artikel

Journal

Catalysis science & technology

Band

Seiten

6496-6502

Anzahl der Seiten

ISSN

2044-4753

Publikationsdatum

2024

Publikationsstatus

Veröffentlicht

Peer-reviewed

Elektronische Version(en)

https://doi.org/10.1039/d4cy00869c (Zugang: Geschlossen)

BibTeX

@article{bf247c240eb34ae4b2be505c5dc2c484,
title = "Unspecific peroxygenase immobilization in 3D-printed microfluidics: towards tailor-made screening platforms",
abstract = "In the context of empowering biocatalysis, an easy-to-develop, reproducible, and easy-to-scale biocatalytic system under continuous flow is demonstrated. 3D printing technology is used as the reactor manufacturing method, yielding identical and low-cost microfluidic chips that can be further modified to serve as a biocatalytic platform for simultaneous parameter screening. The model enzyme studied here is unspecific peroxygenase (UPO). UPOs are currently under intensive study, due to their distinct promiscuity in oxyfunctionalization chemistry. This is the first study demonstrating UPO's immobilization in a microfluidic concept. The developed method for surface functionalization of microfluidic reactors is based on polydopamine modification and was proven highly reproducible. UPO showed a TTN of 19 249 and a STY of 2.1 g L -1 h -1, under the specified conditions. The kinetic behavior of the system under flow conditions is reported. The system was also regenerated with a 51.4% recovered activity. Further utilization of microfluidic concepts is expected to unravel the full potential of UPOs for oxyfunctionalization reactions of particular interest. The proposed system is foreseen as a screening platform for different reaction conditions, reaction substrates, or enzyme mutants.",
author = "Elena Gkantzou and Theofilia Koulopoulou and Hannah Brass and David Sch{\"o}nauer and Anton Glieder and Selin Kara",
note = "Publisher Copyright: {\textcopyright} 2024 The Royal Society of Chemistry.",
year = "2024",
doi = "10.1039/d4cy00869c",
language = "English",
volume = "14",
pages = "6496--6502",
journal = "Catalysis science & technology",
issn = "2044-4753",
publisher = "Royal Society of Chemistry",
number = "22",
}