The 32-kilobase exp gene cluster of Rhizobium meliloti directing the biosynthesis of galactoglucan

Genetic organization and properties of the encoded gene products

verfasst von: Anke Becker, Silvia Rüberg, Helge Küster, Andreas A. Roxlau, Mathias Keller, Tanja Ivashina, Hai Ping Cheng, Graham C. Walker, Alfred Pühler
Abstract: Proteins directing the biosynthesis of galactoglucan (exopolysaccharide II) in Rhizobium meliloti Rm2011 are encoded by the exp genes. Sequence analysis of a 32-kb DNA fragment of megaplasmid 2 containing the exp gene cluster identified previously (J. Glazebrook and G. C. Walker, Cell 56:661- 672, 1989) revealed the presence of 25 open reading frames. Homologies of the deduced exp gene products to proteins of known function suggested that the exp genes encoded four proteins involved in the biosynthesis of dTDP-glucose and dTDP-rhamnose, six glycosyltransferases, an ABC transporter complex homologous to the subfamily of peptide and protein export complexes, and a protein homologous to Rhizobium NodO proteins. In addition, homologies of three Exp proteins to transcriptional regulators, methyltransferases, and periplasmic binding proteins were found. The positions of 26 Tn5 insertions in the exp gene cluster were determined, thus allowing the previously described genetic map to be correlated with the sequence. Operon analysis revealed that the exp gene cluster consists of five complementation groups. In comparison to the wild-type background, all exp complementation groups were transcribed at a substantially elevated level in the regulatory mucR mutant.
Externe Organisation(en): Universität Bielefeld
Russian Academy of Sciences (RAS)
Massachusetts Institute of Technology (MIT)
Typ: Artikel
Journal: Journal of bacteriology
Band: 179
Seiten: 1375-1384
Anzahl der Seiten: 10
ISSN: 0021-9193
Publikationsdatum: 01.01.1997
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
ASJC Scopus Sachgebiete: Mikrobiologie, Molekularbiologie
Elektronische Version(en): https://doi.org/10.1128/jb.179.4.1375-1384.1997 (Zugang: Offen)

BibTeX

@article{b9f6767ff14341cf999231825dd1e618,
title = "The 32-kilobase exp gene cluster of Rhizobium meliloti directing the biosynthesis of galactoglucan: Genetic organization and properties of the encoded gene products",
abstract = "Proteins directing the biosynthesis of galactoglucan (exopolysaccharide II) in Rhizobium meliloti Rm2011 are encoded by the exp genes. Sequence analysis of a 32-kb DNA fragment of megaplasmid 2 containing the exp gene cluster identified previously (J. Glazebrook and G. C. Walker, Cell 56:661- 672, 1989) revealed the presence of 25 open reading frames. Homologies of the deduced exp gene products to proteins of known function suggested that the exp genes encoded four proteins involved in the biosynthesis of dTDP-glucose and dTDP-rhamnose, six glycosyltransferases, an ABC transporter complex homologous to the subfamily of peptide and protein export complexes, and a protein homologous to Rhizobium NodO proteins. In addition, homologies of three Exp proteins to transcriptional regulators, methyltransferases, and periplasmic binding proteins were found. The positions of 26 Tn5 insertions in the exp gene cluster were determined, thus allowing the previously described genetic map to be correlated with the sequence. Operon analysis revealed that the exp gene cluster consists of five complementation groups. In comparison to the wild-type background, all exp complementation groups were transcribed at a substantially elevated level in the regulatory mucR mutant.",
author = "Anke Becker and Silvia R{\"u}berg and Helge K{\"u}ster and Roxlau, {Andreas A.} and Mathias Keller and Tanja Ivashina and Cheng, {Hai Ping} and Walker, {Graham C.} and Alfred P{\"u}hler",
year = "1997",
month = jan,
day = "1",
doi = "10.1128/jb.179.4.1375-1384.1997",
language = "English",
volume = "179",
pages = "1375--1384",
journal = "Journal of bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "4",
}