Biochemical characterization of a molecular switch involving the heat shock protein ClpC, which controls the activity of ComK, the competence transcription factor of Bacillus subtilis

verfasst von: Kürşad Turgay, Leendert W. Hamoen, Gerard Venema, David Dubnau
Abstract: Development of genetic competence in Bacillus subtilis is controlled by the competence-specific transcription factor ComK. ComK activates transcription of itself and several other genes required for competence. The activity of ComK is controlled by other genes including mecA, clpC, and comS. We have used purified ComK, MecA, ClpC, and synthetic ComS to study their interactions and have demonstrated the following mechanism for ComK regulation. ClpC, in the presence of ATP, forms a ternary complex with MecA and ComK, which prevents ComK from binding to its specific DNA target. This complex dissociates when ComS is added, liberating active ComK. ClpC and MecA function as a molecular switch, in which MecA confers molecular recognition, connecting ClpC to ComK and to ComS.
Externe Organisation(en): Public Health Research Institute - New York
Reichsuniversität Groningen
Typ: Artikel
Journal: Genes and Development
Band: 11
Seiten: 119-128
Anzahl der Seiten: 10
ISSN: 0890-9369
Publikationsdatum: 01.01.1997
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
ASJC Scopus Sachgebiete: Genetik, Entwicklungsbiologie
Elektronische Version(en): https://doi.org/10.1101/gad.11.1.119 (Zugang: Offen)

BibTeX

@article{b99a7822d0c94c8c9ad9898b1ec602a8,
title = "Biochemical characterization of a molecular switch involving the heat shock protein ClpC, which controls the activity of ComK, the competence transcription factor of Bacillus subtilis",
abstract = "Development of genetic competence in Bacillus subtilis is controlled by the competence-specific transcription factor ComK. ComK activates transcription of itself and several other genes required for competence. The activity of ComK is controlled by other genes including mecA, clpC, and comS. We have used purified ComK, MecA, ClpC, and synthetic ComS to study their interactions and have demonstrated the following mechanism for ComK regulation. ClpC, in the presence of ATP, forms a ternary complex with MecA and ComK, which prevents ComK from binding to its specific DNA target. This complex dissociates when ComS is added, liberating active ComK. ClpC and MecA function as a molecular switch, in which MecA confers molecular recognition, connecting ClpC to ComK and to ComS.",
keywords = "ClpC, ComK, competence, heat shock, HSP100, MecA",
author = "K{\"u}r{\c s}ad Turgay and Hamoen, {Leendert W.} and Gerard Venema and David Dubnau",
note = "Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",
year = "1997",
month = jan,
day = "1",
doi = "10.1101/gad.11.1.119",
language = "English",
volume = "11",
pages = "119--128",
journal = "Genes and Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "1",
}