Birch pollen-induced signatures in dendritic cells are maintained upon additional cytomegalovirus exposure

verfasst von: Zeinab Fneish, Jennifer Becker, Felix Mulenge, Firas Fneish, Bibiana Costa, Claudia Traidl-Hoffmann, Stefanie Gilles, Ulrich Kalinke
Abstract: During the birch pollen season an enhanced incidence of virus infections is noticed, raising the question whether pollen can affect anti-viral responses independent of allergic reactions. We previously showed that birch pollen-treatment of monocyte-derived dendritic cells (moDC) enhances human cytomegalovirus (HCMV) infection. Here we addressed how in moDC the relatively weak pollen response can affect the comparably strong response to HCMV. To this end, moDC were stimulated with aqueous birch pollen extract (APE), HCMV, and APE with HCMV, and transcriptomic signatures were determined after 6 and 24 h of incubation. Infection was monitored upon exposure of moDC to GFP expressing HCMV by flow cytometric analysis of GFP expressing cells. Principle component analysis of RNA sequencing data revealed close clustering of mock and APE treated moDC, whereas HCMV as well as APE with HCMV treated moDC clustered separately after 6 and 24 h of incubation, respectively. Communally induced genes were detected in APE, HCMV and APE with HCMV treated moDC. In APE with HCMV treated moDC, the comparably weak APE induced signatures were maintained after HCMV exposure. In particular, NF-κB/RELA and PI3K/AKT/MAPK signaling were altered upon APE with HCMV exposure. Earlier, we discovered that NF-κB inhibition alleviated APE induced enhancement of HCMV infection. Here we additionally found that impairment of PI3K signaling reduced HCMV infection in HCMV and APE with HCMV treated moDC. APE treated moDC that were exposed to HCMV show a unique host gene signature, which to a large extent is regulated by NF-κB activation and PI3K/AKT/MAPK signaling.
Organisationseinheit(en): Institut für Zellbiologie und Biophysik
Externe Organisation(en): TWINCORE Zentrum für Experimentelle und Klinische Infektionsforschung GmbH
Universität Augsburg
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt
Christine Kühne – Center for Allergy Research and Education (CK-CARE)
Medizinische Hochschule Hannover (MHH)
Typ: Kurzmitteilung
Journal: GENE
Band: 927
Anzahl der Seiten: 7
ISSN: 0378-1119
Publikationsdatum: 15.11.2024
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
ASJC Scopus Sachgebiete: Genetik
Elektronische Version(en): https://doi.org/10.1016/j.gene.2024.148649 (Zugang: Offen)

BibTeX

@article{ad758688b9984f9f9d8bf5024bfce604,
title = "Birch pollen-induced signatures in dendritic cells are maintained upon additional cytomegalovirus exposure",
abstract = "During the birch pollen season an enhanced incidence of virus infections is noticed, raising the question whether pollen can affect anti-viral responses independent of allergic reactions. We previously showed that birch pollen-treatment of monocyte-derived dendritic cells (moDC) enhances human cytomegalovirus (HCMV) infection. Here we addressed how in moDC the relatively weak pollen response can affect the comparably strong response to HCMV. To this end, moDC were stimulated with aqueous birch pollen extract (APE), HCMV, and APE with HCMV, and transcriptomic signatures were determined after 6 and 24 h of incubation. Infection was monitored upon exposure of moDC to GFP expressing HCMV by flow cytometric analysis of GFP expressing cells. Principle component analysis of RNA sequencing data revealed close clustering of mock and APE treated moDC, whereas HCMV as well as APE with HCMV treated moDC clustered separately after 6 and 24 h of incubation, respectively. Communally induced genes were detected in APE, HCMV and APE with HCMV treated moDC. In APE with HCMV treated moDC, the comparably weak APE induced signatures were maintained after HCMV exposure. In particular, NF-κB/RELA and PI3K/AKT/MAPK signaling were altered upon APE with HCMV exposure. Earlier, we discovered that NF-κB inhibition alleviated APE induced enhancement of HCMV infection. Here we additionally found that impairment of PI3K signaling reduced HCMV infection in HCMV and APE with HCMV treated moDC. APE treated moDC that were exposed to HCMV show a unique host gene signature, which to a large extent is regulated by NF-κB activation and PI3K/AKT/MAPK signaling.",
keywords = "Antiviral immunity, Aqueous birch pollen extract, Monocyte-derived dendritic cells, PI3K signaling, Pro-inflammatory cytokines, RNA-sequencing, Viral infection",
author = "Zeinab Fneish and Jennifer Becker and Felix Mulenge and Firas Fneish and Bibiana Costa and Claudia Traidl-Hoffmann and Stefanie Gilles and Ulrich Kalinke",
note = "Publisher Copyright: {\textcopyright} 2024 The Author(s)",
year = "2024",
month = nov,
day = "15",
doi = "10.1016/j.gene.2024.148649",
language = "English",
volume = "927",
journal = "GENE",
issn = "0378-1119",
publisher = "Elsevier",
}