Mechanical strain using 2D and 3D bioreactors induces osteogenesis

implications for bone tissue engineering

verfasst von: M van Griensven, S Diederichs, S Roeker, S Boehm, A Peterbauer, S Wolbank, D Riechers, F Stahl, C Kasper
Abstract: Fracture healing is a complicated process involving many growth factors, cells, and physical forces. In cases, where natural healing is not able, efforts have to be undertaken to improve healing. For this purpose, tissue engineering may be an option. In order to stimulate cells to form a bone tissue several factors are needed: cells, scaffold, and growth factors. Stem cells derived from bone marrow or adipose tissues are the most useful in this regard. The differentiation of the cells can be accelerated using mechanical stimulation. The first part of this chapter describes the influence of longitudinal strain application. The second part uses a sophisticated approach with stem cells on a newly developed biomaterial (Sponceram) in a rotating bed bioreactor with the administration of bone morphogenetic protein-2. It is shown that such an approach is able to produce bone tissue constructs. This may lead to production of larger constructs that can be used in clinical applications.
Organisationseinheit(en): Institut für Technische Chemie
Externe Organisation(en): Ludwig Boltzmann Institute for Experimental and Clinical Traumatology
Typ: Artikel
Journal: Advances in Biochemical Engineering/Biotechnology
Band: 112
Seiten: 95-123
Anzahl der Seiten: 29
ISSN: 0724-6145
Publikationsdatum: 2009
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
Elektronische Version(en): https://doi.org/10.1007/978-3-540-69357-4_5 (Zugang: Unbekannt)

BibTeX

@article{aa3de019b34549ef8a6d55854952a65b,
title = "Mechanical strain using 2D and 3D bioreactors induces osteogenesis: implications for bone tissue engineering",
abstract = "Fracture healing is a complicated process involving many growth factors, cells, and physical forces. In cases, where natural healing is not able, efforts have to be undertaken to improve healing. For this purpose, tissue engineering may be an option. In order to stimulate cells to form a bone tissue several factors are needed: cells, scaffold, and growth factors. Stem cells derived from bone marrow or adipose tissues are the most useful in this regard. The differentiation of the cells can be accelerated using mechanical stimulation. The first part of this chapter describes the influence of longitudinal strain application. The second part uses a sophisticated approach with stem cells on a newly developed biomaterial (Sponceram) in a rotating bed bioreactor with the administration of bone morphogenetic protein-2. It is shown that such an approach is able to produce bone tissue constructs. This may lead to production of larger constructs that can be used in clinical applications.",
keywords = "Biomechanical Phenomena, Bioreactors, Bone Marrow Cells/cytology, Bone Morphogenetic Protein 2/metabolism, Bone and Bones/cytology, Cell Differentiation, Equipment Design, Humans, Intercellular Signaling Peptides and Proteins/metabolism, Mechanotransduction, Cellular, Osteogenesis, Stem Cells/cytology, Tissue Culture Techniques/instrumentation, Tissue Engineering/instrumentation, Tissue Scaffolds",
author = "{van Griensven}, M and S Diederichs and S Roeker and S Boehm and A Peterbauer and S Wolbank and D Riechers and F Stahl and C Kasper",
note = "Funding information: This work was partially carried out under the scope of the European NoE ?EXPERTISSUES? (NMP3-CT-2004-500283). Solvig Diederichs was a student in the early research training program Marie Curie ?Alea Jacta EST? (MEST-CT-2004-8104). The Sponceram ceramics were kindly provided by Zellwerk GmbH (Oberkr?mer, Germany) and the BMP-2 was a gift from Prof. Walter Sebald (University of W?rzburg).",
year = "2009",
doi = "10.1007/978-3-540-69357-4_5",
language = "English",
volume = "112",
pages = "95--123",
journal = "Advances in Biochemical Engineering/Biotechnology",
issn = "0724-6145",
publisher = "Springer Science and Business Media Deutschland GmbH",
}