Flow cytometric analyses in embryogenic and non-embryogenic callus lines of Cyclamen persicum mill.

Relation between ploidy level and competence for somatic embryogenesis

verfasst von: T. Winkelmann, R. S. Sangwan, H. G. Schwenkel
Abstract: Embryogenic and non-embryogenic callus lines derived from the same diploid Cyclamen persicum genotype ('Purple Flamed') were analyzed by flow cytometry and compared to the initial plant material. The DNA content of the diploid plant in the greenhouse was 1.12 pg DNA/2C as estimated in relation to the internal standards tomato nuclei and chicken erythrocytes. In both callus lines the majority of cells contained the same amount of DNA as the initial plant, indicating that no polyploidization has taken place after 5 years of culture on medium containing 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.8 mg/l 6-(γ-γ-dimethylallylamino)purine(zip). Thus, our data suggest that in Cyclamen callus lines there was no strict correlation between the ploidy level and the ability to produce somatic embryos. Furthermore, following the proportion of cells in the three phases of the cell cycle (G0/GI, S, G2/M) during one subculture period of 4 weeks revealed high division activity within the first 2 weeks for both callus lines cultured on the 2,4-D-containing medium. However, when transferred to hormone-free medium, the division activity of the embryogenic cell line decreased markedly, corresponding to the differentiation of somatic embryos. In contrast, for the non-embryogenic callus an increase in cells in the G2/M phase was observed.
Organisationseinheit(en): Abteilung Gehölz- und Vermehrungsphysiologie
Institut für Gartenbauliche Produktionssysteme
Externe Organisation(en): Universite de Picardie Jules Verne
Typ: Artikel
Journal: Plant cell reports
Band: 17
Seiten: 400-404
Anzahl der Seiten: 5
ISSN: 0721-7714
Publikationsdatum: 03.1998
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
ASJC Scopus Sachgebiete: Agronomie und Nutzpflanzenwissenschaften, Pflanzenkunde
Elektronische Version(en): https://doi.org/10.1007/s002990050414 (Zugang: Geschlossen)

BibTeX

@article{5a1e058c9ba849a390707f849f748ac9,
title = "Flow cytometric analyses in embryogenic and non-embryogenic callus lines of Cyclamen persicum mill.: Relation between ploidy level and competence for somatic embryogenesis",
abstract = "Embryogenic and non-embryogenic callus lines derived from the same diploid Cyclamen persicum genotype ('Purple Flamed') were analyzed by flow cytometry and compared to the initial plant material. The DNA content of the diploid plant in the greenhouse was 1.12 pg DNA/2C as estimated in relation to the internal standards tomato nuclei and chicken erythrocytes. In both callus lines the majority of cells contained the same amount of DNA as the initial plant, indicating that no polyploidization has taken place after 5 years of culture on medium containing 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.8 mg/l 6-(γ-γ-dimethylallylamino)purine(zip). Thus, our data suggest that in Cyclamen callus lines there was no strict correlation between the ploidy level and the ability to produce somatic embryos. Furthermore, following the proportion of cells in the three phases of the cell cycle (G0/GI, S, G2/M) during one subculture period of 4 weeks revealed high division activity within the first 2 weeks for both callus lines cultured on the 2,4-D-containing medium. However, when transferred to hormone-free medium, the division activity of the embryogenic cell line decreased markedly, corresponding to the differentiation of somatic embryos. In contrast, for the non-embryogenic callus an increase in cells in the G2/M phase was observed.",
keywords = "Cell cycle, Cyclamen persicum, Flow cytometry, Somatic embryogenesis",
author = "T. Winkelmann and Sangwan, {R. S.} and Schwenkel, {H. G.}",
year = "1998",
month = mar,
doi = "10.1007/s002990050414",
language = "English",
volume = "17",
pages = "400--404",
journal = "Plant cell reports",
issn = "0721-7714",
publisher = "Springer Verlag",
number = "5",
}