New 3-D microarray platform based on macroporous polymer monoliths

verfasst von: M Rober, J Walter, E Vlakh, F Stahl, C Kasper, T Tennikova
Abstract: Polymer macroporous monoliths are widely used as efficient sorbents in different, mostly dynamic, interphase processes. In this paper, monolithic materials strongly bound to the inert glass surface are suggested as operative matrices at the development of three-dimensional (3-D) microarrays. For this purpose, several rigid macroporous copolymers differed by reactivity and hydrophobic-hydrophilic properties were synthesized and tested: (1) glycidyl methacrylate-co-ethylene dimethacrylate (poly(GMA-co-EDMA)), (2) glycidyl methacrylate-co-glycerol dimethacrylate (poly(GMA-co-GDMA)), (3) N-hydroxyphthalimide ester of acrylic acid-co-glycidyl methacrylate-co-ethylene dimethacrylate (poly(HPIEAA-co-GMA-co-EDMA)), (4) 2-cyanoethyl methacrylate-co-ethylene dimethacrylate (poly(CEMA-co-EDMA)), and (5) 2-cyanoethyl methacrylate-co-2-hydroxyethyl methacrylate-co-ethylene dimethacrylate (poly(CEMA-co-HEMA-co-EDMA)). The constructed devices were used as platforms for protein microarrays construction and model mouse IgG-goat anti-mouse IgG affinity pair was used to demonstrate the potential of developed test-systems, as well as to optimize microanalytical conditions. The offered microarray platforms were applied to detect the bone tissue marker osteopontin directly in cell culture medium.
Organisationseinheit(en): Institut für Technische Chemie
Externe Organisation(en): Institute for Analytical Instrumentation of the Russian Academy of Sciences
Typ: Artikel
Journal: Analytica chimica acta
Band: 644
Seiten: 95-103
Anzahl der Seiten: 9
ISSN: 0003-2670
Publikationsdatum: 30.06.2009
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
Elektronische Version(en): https://doi.org/10.1016/j.aca.2009.04.015 (Zugang: Unbekannt)

BibTeX

@article{29d9bb0484704151a6ae7279d052d873,
title = "New 3-D microarray platform based on macroporous polymer monoliths",
abstract = "Polymer macroporous monoliths are widely used as efficient sorbents in different, mostly dynamic, interphase processes. In this paper, monolithic materials strongly bound to the inert glass surface are suggested as operative matrices at the development of three-dimensional (3-D) microarrays. For this purpose, several rigid macroporous copolymers differed by reactivity and hydrophobic-hydrophilic properties were synthesized and tested: (1) glycidyl methacrylate-co-ethylene dimethacrylate (poly(GMA-co-EDMA)), (2) glycidyl methacrylate-co-glycerol dimethacrylate (poly(GMA-co-GDMA)), (3) N-hydroxyphthalimide ester of acrylic acid-co-glycidyl methacrylate-co-ethylene dimethacrylate (poly(HPIEAA-co-GMA-co-EDMA)), (4) 2-cyanoethyl methacrylate-co-ethylene dimethacrylate (poly(CEMA-co-EDMA)), and (5) 2-cyanoethyl methacrylate-co-2-hydroxyethyl methacrylate-co-ethylene dimethacrylate (poly(CEMA-co-HEMA-co-EDMA)). The constructed devices were used as platforms for protein microarrays construction and model mouse IgG-goat anti-mouse IgG affinity pair was used to demonstrate the potential of developed test-systems, as well as to optimize microanalytical conditions. The offered microarray platforms were applied to detect the bone tissue marker osteopontin directly in cell culture medium.",
keywords = "Animals, Goats, Immunoglobulin G/chemistry, Mice, Osteopontin/analysis, Polymers/chemical synthesis, Protein Array Analysis/methods",
author = "M Rober and J Walter and E Vlakh and F Stahl and C Kasper and T Tennikova",
note = "Funding information: This work was supported by DFG grant (Grant No. KA 1784/4-1; Coordinators: Prof. T. Tennikova, IMC RAS and Dr. C. Kasper, ITC UH) and RBRF grant (08-08-00876-a, Supervisor Prof. T. Tennikova). The authors are grateful to German Academic Exchange Service (DAAD) for providing the scholarship for PhD student Marina Rober for four-month work at the Institute of Technical Chemistry, University of Hannover (ITC UH). The authors are also grateful to Prof. Thomas Scheper (ITC, Hannover University) and Prof. Valery Krasikov (IMC RAS) for organizing help and fruitful discussions.",
year = "2009",
month = jun,
day = "30",
doi = "10.1016/j.aca.2009.04.015",
language = "English",
volume = "644",
pages = "95--103",
journal = "Analytica chimica acta",
issn = "0003-2670",
publisher = "Elsevier",
number = "1-2",
}